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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium sp. 50S ribosomal protein L15 (rplO) CSB-YP399287BPE
CSB-EP399287BPE
CSB-BP399287BPE
CSB-MP399287BPE
CSB-EP399287BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L6 (rplF) CSB-YP399288BPE
CSB-EP399288BPE
CSB-BP399288BPE
CSB-MP399288BPE
CSB-EP399288BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L4 (rplD) CSB-YP399289BPE
CSB-EP399289BPE
CSB-BP399289BPE
CSB-MP399289BPE
CSB-EP399289BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S12 (rpsL) CSB-YP399290BPE
CSB-EP399290BPE
CSB-BP399290BPE
CSB-MP399290BPE
CSB-EP399290BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L10 (rplJ) CSB-YP399291BPE
CSB-EP399291BPE
CSB-BP399291BPE
CSB-MP399291BPE
CSB-EP399291BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Alanine--tRNA ligase (alaS), partial CSB-YP399292BPE
CSB-EP399292BPE
CSB-BP399292BPE
CSB-MP399292BPE
CSB-EP399292BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphoheptose isomerase (gmhA) CSB-YP399293BPE
CSB-EP399293BPE
CSB-BP399293BPE
CSB-MP399293BPE
CSB-EP399293BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP399294BPE
CSB-EP399294BPE
CSB-BP399294BPE
CSB-MP399294BPE
CSB-EP399294BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0391 membrane protein BBta_6140 (BBta_6140), partial CSB-YP399295BPE
CSB-EP399295BPE
CSB-BP399295BPE
CSB-MP399295BPE
CSB-EP399295BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP399296BPE
CSB-EP399296BPE
CSB-BP399296BPE
CSB-MP399296BPE
CSB-EP399296BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphomethylpyrimidine synthase (thiC), partial CSB-YP399297BPE
CSB-EP399297BPE
CSB-BP399297BPE
CSB-MP399297BPE
CSB-EP399297BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Fructose-1,6-bisphosphatase class 1 2 (fbp2) CSB-YP399298BPE
CSB-EP399298BPE
CSB-BP399298BPE
CSB-MP399298BPE
CSB-EP399298BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable 5-dehydro-4-deoxyglucarate dehydratase (BBta_6487) CSB-YP399299BPE
CSB-EP399299BPE
CSB-BP399299BPE
CSB-MP399299BPE
CSB-EP399299BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP399300BPE
CSB-EP399300BPE
CSB-BP399300BPE
CSB-MP399300BPE
CSB-EP399300BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0301 protein BBta_6966 (BBta_6966) CSB-YP399301BPE
CSB-EP399301BPE
CSB-BP399301BPE
CSB-MP399301BPE
CSB-EP399301BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urease accessory protein UreD 3 (ureD3) CSB-YP399302BPE
CSB-EP399302BPE
CSB-BP399302BPE
CSB-MP399302BPE
CSB-EP399302BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Acetylglutamate kinase (argB) CSB-YP399303BPE
CSB-EP399303BPE
CSB-BP399303BPE
CSB-MP399303BPE
CSB-EP399303BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0133 protein BBta_7345 (BBta_7345) CSB-YP399304BPE
CSB-EP399304BPE
CSB-BP399304BPE
CSB-MP399304BPE
CSB-EP399304BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP399305BPE
CSB-EP399305BPE
CSB-BP399305BPE
CSB-MP399305BPE
CSB-EP399305BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L32 (rpmF) CSB-YP399306BPE
CSB-EP399306BPE
CSB-BP399306BPE
CSB-MP399306BPE
CSB-EP399306BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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