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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lodderomyces elongisporus Restriction of telomere capping protein 5 (RTC5), partial CSB-YP399227LLV
CSB-EP399227LLV
CSB-BP399227LLV
CSB-MP399227LLV
CSB-EP399227LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus WD repeat-containing protein JIP5 (JIP5), partial CSB-YP399228LLV
CSB-EP399228LLV
CSB-BP399228LLV
CSB-MP399228LLV
CSB-EP399228LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP399229LLV
CSB-EP399229LLV
CSB-BP399229LLV
CSB-MP399229LLV
CSB-EP399229LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Ubiquitin-related modifier 1 (URM1) CSB-YP399230LLV
CSB-EP399230LLV
CSB-BP399230LLV
CSB-MP399230LLV
CSB-EP399230LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus U1 small nuclear ribonucleoprotein component SNU71 (SNU71), partial CSB-YP399231LLV
CSB-EP399231LLV
CSB-BP399231LLV
CSB-MP399231LLV
CSB-EP399231LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Mitochondrial distribution and morphology protein 10 (MDM10), partial CSB-YP399232LLV
CSB-EP399232LLV
CSB-BP399232LLV
CSB-MP399232LLV
CSB-EP399232LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus UPF0507 protein LELG_01076 (LELG_01076), partial CSB-YP399233LLV
CSB-EP399233LLV
CSB-BP399233LLV
CSB-MP399233LLV
CSB-EP399233LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Actin cytoskeleton-regulatory complex protein PAN1 (PAN1), partial CSB-YP399234LLV
CSB-EP399234LLV
CSB-BP399234LLV
CSB-MP399234LLV
CSB-EP399234LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Autophagy-related protein 3 (ATG3) CSB-YP399235LLV
CSB-EP399235LLV
CSB-BP399235LLV
CSB-MP399235LLV
CSB-EP399235LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Autophagy-related protein 18 (ATG18), partial CSB-YP399236LLV
CSB-EP399236LLV
CSB-BP399236LLV
CSB-MP399236LLV
CSB-EP399236LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Ribosome biogenesis protein ERB1 (ERB1), partial CSB-YP399237LLV
CSB-EP399237LLV
CSB-BP399237LLV
CSB-MP399237LLV
CSB-EP399237LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Protein TIF31 homolog (TIF31), partial CSB-YP399238LLV
CSB-EP399238LLV
CSB-BP399238LLV
CSB-MP399238LLV
CSB-EP399238LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Putative transferase CAF17, mitochondrial (CAF17) CSB-YP399239LLV
CSB-EP399239LLV
CSB-BP399239LLV
CSB-MP399239LLV
CSB-EP399239LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Protein CLP1 (CLP1), partial CSB-YP399240LLV
CSB-EP399240LLV
CSB-BP399240LLV
CSB-MP399240LLV
CSB-EP399240LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Required for respiratory growth protein 9, mitochondrial (RRG9), partial CSB-YP399241LLV
CSB-EP399241LLV
CSB-BP399241LLV
CSB-MP399241LLV
CSB-EP399241LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Increased rDNA silencing protein 4 (IRS4), partial CSB-YP399242LLV
CSB-EP399242LLV
CSB-BP399242LLV
CSB-MP399242LLV
CSB-EP399242LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Metacaspase-1 (MCA1), partial CSB-YP399243LLV
CSB-EP399243LLV
CSB-BP399243LLV
CSB-MP399243LLV
CSB-EP399243LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Riboflavin kinase (FMN1) CSB-YP399244LLV
CSB-EP399244LLV
CSB-BP399244LLV
CSB-MP399244LLV
CSB-EP399244LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus U3 small nucleolar RNA-associated protein 10 (UTP10), partial CSB-YP399245LLV
CSB-EP399245LLV
CSB-BP399245LLV
CSB-MP399245LLV
CSB-EP399245LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Altered inheritance of mitochondria protein 31, mitochondrial (AIM31), partial CSB-YP399246LLV1
CSB-EP399246LLV1
CSB-BP399246LLV1
CSB-MP399246LLV1
CSB-EP399246LLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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