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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Meyerozyma guilliermondii Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP399487MTM
CSB-EP399487MTM
CSB-BP399487MTM
CSB-MP399487MTM
CSB-EP399487MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Protein transport protein SEC31 (SEC31), partial CSB-YP399488MTM
CSB-EP399488MTM
CSB-BP399488MTM
CSB-MP399488MTM
CSB-EP399488MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii tRNA-dihydrouridine (47) synthase [NAD (P) (+)], partial CSB-YP399489MTM
CSB-EP399489MTM
CSB-BP399489MTM
CSB-MP399489MTM
CSB-EP399489MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Protein RMD9, mitochondrial (RMD9), partial CSB-YP399490MTM
CSB-EP399490MTM
CSB-BP399490MTM
CSB-MP399490MTM
CSB-EP399490MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Putative tyrosine-protein phosphatase OCA1 (OCA1) CSB-YP399491MTM
CSB-EP399491MTM
CSB-BP399491MTM
CSB-MP399491MTM
CSB-EP399491MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Protein SQS1 (SQS1), partial CSB-YP399492MTM
CSB-EP399492MTM
CSB-BP399492MTM
CSB-MP399492MTM
CSB-EP399492MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase MSS116, mitochondrial (MSS116), partial CSB-YP399493MTM
CSB-EP399493MTM
CSB-BP399493MTM
CSB-MP399493MTM
CSB-EP399493MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Leukotriene A-4 hydrolase homolog 2 (PGUG_02354), partial CSB-YP399494MTM
CSB-EP399494MTM
CSB-BP399494MTM
CSB-MP399494MTM
CSB-EP399494MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP399495MTM
CSB-EP399495MTM
CSB-BP399495MTM
CSB-MP399495MTM
CSB-EP399495MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Mitochondrial outer membrane protein IML2 (IML2), partial CSB-YP399496MTM
CSB-EP399496MTM
CSB-BP399496MTM
CSB-MP399496MTM
CSB-EP399496MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Topoisomerase I damage affected protein 11 (TDA11) CSB-YP399497MTM
CSB-EP399497MTM
CSB-BP399497MTM
CSB-MP399497MTM
CSB-EP399497MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Altered inheritance of mitochondria protein 31, mitochondrial (AIM31), partial CSB-YP399498MTM1
CSB-EP399498MTM1
CSB-BP399498MTM1
CSB-MP399498MTM1
CSB-EP399498MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase DHH1 (DHH1), partial CSB-YP399499MTM
CSB-EP399499MTM
CSB-BP399499MTM
CSB-MP399499MTM
CSB-EP399499MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Mitochondrial thiamine pyrophosphate carrier 1 (TPC1), partial CSB-YP399500MTM1
CSB-EP399500MTM1
CSB-BP399500MTM1
CSB-MP399500MTM1
CSB-EP399500MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Histone H2B.1 (HTB1) CSB-YP399501MTM
CSB-EP399501MTM
CSB-BP399501MTM
CSB-MP399501MTM
CSB-EP399501MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Dol-P-Man:Man (5)GlcNAc (2)-PP-Dol alpha-1,3-mannosyltransferase, partial CSB-YP399502MTM
CSB-EP399502MTM
CSB-BP399502MTM
CSB-MP399502MTM
CSB-EP399502MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Nuclear distribution protein PAC1 (PAC1), partial CSB-YP399503MTM
CSB-EP399503MTM
CSB-BP399503MTM
CSB-MP399503MTM
CSB-EP399503MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Ribosome biogenesis protein NSA1 (NSA1) CSB-YP399504MTM
CSB-EP399504MTM
CSB-BP399504MTM
CSB-MP399504MTM
CSB-EP399504MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase DBP4 (DBP4), partial CSB-YP399505MTM
CSB-EP399505MTM
CSB-BP399505MTM
CSB-MP399505MTM
CSB-EP399505MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Increased recombination centers protein 6 (IRC6) CSB-YP399506MTM
CSB-EP399506MTM
CSB-BP399506MTM
CSB-MP399506MTM
CSB-EP399506MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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