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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio cholerae serotype O1 Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP399407VEY
CSB-EP399407VEY
CSB-BP399407VEY
CSB-MP399407VEY
CSB-EP399407VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Sugar fermentation stimulation protein homolog (sfsA) CSB-YP399408VEY
CSB-EP399408VEY
CSB-BP399408VEY
CSB-MP399408VEY
CSB-EP399408VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 50S ribosomal protein L13 (rplM) CSB-YP399409VEY
CSB-EP399409VEY
CSB-BP399409VEY
CSB-MP399409VEY
CSB-EP399409VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Carbon storage regulator homolog (csrA) CSB-YP399410VEY
CSB-EP399410VEY
CSB-BP399410VEY
CSB-MP399410VEY
CSB-EP399410VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP399411VEY
CSB-EP399411VEY
CSB-BP399411VEY
CSB-MP399411VEY
CSB-EP399411VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Phosphoglycerate kinase (pgk) CSB-YP399412VEY
CSB-EP399412VEY
CSB-BP399412VEY
CSB-MP399412VEY
CSB-EP399412VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0235 protein VC0395_A0010/VC395_0502 (VC0395_A0010, VC395_0502) CSB-YP399413VEY
CSB-EP399413VEY
CSB-BP399413VEY
CSB-MP399413VEY
CSB-EP399413VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L28 (rpmB) CSB-YP399414FDT
CSB-EP399414FDT
CSB-BP399414FDT
CSB-MP399414FDT
CSB-EP399414FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Tryptophan synthase alpha chain (trpA) CSB-YP399415FDT
CSB-EP399415FDT
CSB-BP399415FDT
CSB-MP399415FDT
CSB-EP399415FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Urease subunit gamma (ureA) CSB-YP399416FDT
CSB-EP399416FDT
CSB-BP399416FDT
CSB-MP399416FDT
CSB-EP399416FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Aspartate carbamoyltransferase (pyrB) CSB-YP399417FDT
CSB-EP399417FDT
CSB-BP399417FDT
CSB-MP399417FDT
CSB-EP399417FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae L-rhamnose mutarotase (rhaM) CSB-YP399418FDT
CSB-EP399418FDT
CSB-BP399418FDT
CSB-MP399418FDT
CSB-EP399418FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP399419FDT
CSB-EP399419FDT
CSB-BP399419FDT
CSB-MP399419FDT
CSB-EP399419FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae tRNA modification GTPase MnmE (mnmE) CSB-YP399420FDT
CSB-EP399420FDT
CSB-BP399420FDT
CSB-MP399420FDT
CSB-EP399420FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae D-alanine--D-alanine ligase (ddl) CSB-YP399421FDT
CSB-EP399421FDT
CSB-BP399421FDT
CSB-MP399421FDT
CSB-EP399421FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Probable malate:quinone oxidoreductase (mqo) CSB-YP399422FDT
CSB-EP399422FDT
CSB-BP399422FDT
CSB-MP399422FDT
CSB-EP399422FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP399423FDT
CSB-EP399423FDT
CSB-BP399423FDT
CSB-MP399423FDT
CSB-EP399423FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L19 (rplS) CSB-YP399424FDT
CSB-EP399424FDT
CSB-BP399424FDT
CSB-MP399424FDT
CSB-EP399424FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Proline iminopeptidase (fpaP) CSB-YP399425FDT
CSB-EP399425FDT
CSB-BP399425FDT
CSB-MP399425FDT
CSB-EP399425FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L10 (rplJ) CSB-YP399426FDT
CSB-EP399426FDT
CSB-BP399426FDT
CSB-MP399426FDT
CSB-EP399426FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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