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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter uraniireducens DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP400807GEI
CSB-EP400807GEI
CSB-BP400807GEI
CSB-MP400807GEI
CSB-EP400807GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP400808GEI
CSB-EP400808GEI
CSB-BP400808GEI
CSB-MP400808GEI
CSB-EP400808GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Homoserine O-acetyltransferase (metX) CSB-YP400809GEI
CSB-EP400809GEI
CSB-BP400809GEI
CSB-MP400809GEI
CSB-EP400809GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L27 (rpmA) CSB-YP400810GEI
CSB-EP400810GEI
CSB-BP400810GEI
CSB-MP400810GEI
CSB-EP400810GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens LL-diaminopimelate aminotransferase (dapL) CSB-YP400811GEI
CSB-EP400811GEI
CSB-BP400811GEI
CSB-MP400811GEI
CSB-EP400811GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Quinolinate synthase A (nadA) CSB-YP400812GEI
CSB-EP400812GEI
CSB-BP400812GEI
CSB-MP400812GEI
CSB-EP400812GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP400813GEI
CSB-EP400813GEI
CSB-BP400813GEI
CSB-MP400813GEI
CSB-EP400813GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens DNA mismatch repair protein mutL (mutL), partial CSB-YP400814GEI
CSB-EP400814GEI
CSB-BP400814GEI
CSB-MP400814GEI
CSB-EP400814GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Aspartate carbamoyltransferase (pyrB) CSB-YP400815GEI
CSB-EP400815GEI
CSB-BP400815GEI
CSB-MP400815GEI
CSB-EP400815GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Ribosome-binding factor A (rbfA) CSB-YP400816GEI
CSB-EP400816GEI
CSB-BP400816GEI
CSB-MP400816GEI
CSB-EP400816GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP400817GEI
CSB-EP400817GEI
CSB-BP400817GEI
CSB-MP400817GEI
CSB-EP400817GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP400818SVC
CSB-EP400818SVC
CSB-BP400818SVC
CSB-MP400818SVC
CSB-EP400818SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor P (efp) CSB-YP400819SVC
CSB-EP400819SVC
CSB-BP400819SVC
CSB-MP400819SVC
CSB-EP400819SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Protein translocase subunit SecA (secA), partial CSB-YP400820SVC
CSB-EP400820SVC
CSB-BP400820SVC
CSB-MP400820SVC
CSB-EP400820SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit K CSB-YP400821SVC
CSB-EP400821SVC
CSB-BP400821SVC
CSB-MP400821SVC
CSB-EP400821SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Sugar fermentation stimulation protein homolog (sfsA) CSB-YP400822SVC
CSB-EP400822SVC
CSB-BP400822SVC
CSB-MP400822SVC
CSB-EP400822SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoglucosamine mutase (glmM) CSB-YP400823SVC
CSB-EP400823SVC
CSB-BP400823SVC
CSB-MP400823SVC
CSB-EP400823SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem Q (B) protein 1, partial CSB-YP400824SVC1
CSB-EP400824SVC1
CSB-BP400824SVC1
CSB-MP400824SVC1
CSB-EP400824SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S11 (rpsK) CSB-YP400825SVC
CSB-EP400825SVC
CSB-BP400825SVC
CSB-MP400825SVC
CSB-EP400825SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L18 (rplR) CSB-YP400826SVC
CSB-EP400826SVC
CSB-BP400826SVC
CSB-MP400826SVC
CSB-EP400826SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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