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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter uraniireducens Peptidyl-tRNA hydrolase (pth) CSB-YP402208GEI
CSB-EP402208GEI
CSB-BP402208GEI
CSB-MP402208GEI
CSB-EP402208GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Ketol-acid reductoisomerase (ilvC) CSB-YP402209GEI
CSB-EP402209GEI
CSB-BP402209GEI
CSB-MP402209GEI
CSB-EP402209GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens NH (3)-dependent NAD (+) synthetase CSB-YP402210GEI
CSB-EP402210GEI
CSB-BP402210GEI
CSB-MP402210GEI
CSB-EP402210GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens tRNA (guanine-N (1)-)-methyltransferase CSB-YP402211GEI
CSB-EP402211GEI
CSB-BP402211GEI
CSB-MP402211GEI
CSB-EP402211GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP402212GEI
CSB-EP402212GEI
CSB-BP402212GEI
CSB-MP402212GEI
CSB-EP402212GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP402213GEI
CSB-EP402213GEI
CSB-BP402213GEI
CSB-MP402213GEI
CSB-EP402213GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UPF0758 protein Gura_4138 (Gura_4138) CSB-YP402214GEI
CSB-EP402214GEI
CSB-BP402214GEI
CSB-MP402214GEI
CSB-EP402214GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP402215GEI
CSB-EP402215GEI
CSB-BP402215GEI
CSB-MP402215GEI
CSB-EP402215GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP402216GEI
CSB-EP402216GEI
CSB-BP402216GEI
CSB-MP402216GEI
CSB-EP402216GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP402217GEI
CSB-EP402217GEI
CSB-BP402217GEI
CSB-MP402217GEI
CSB-EP402217GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens tRNA modification GTPase MnmE (mnmE) CSB-YP402218GEI
CSB-EP402218GEI
CSB-BP402218GEI
CSB-MP402218GEI
CSB-EP402218GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP402219GEI
CSB-EP402219GEI
CSB-BP402219GEI
CSB-MP402219GEI
CSB-EP402219GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens FMN-dependent NADH-azoreductase (azoR) CSB-YP402220GEI
CSB-EP402220GEI
CSB-BP402220GEI
CSB-MP402220GEI
CSB-EP402220GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Ribonuclease Y (rny), partial CSB-YP402221GEI
CSB-EP402221GEI
CSB-BP402221GEI
CSB-MP402221GEI
CSB-EP402221GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L5 (rplE) CSB-YP402222GEI
CSB-EP402222GEI
CSB-BP402222GEI
CSB-MP402222GEI
CSB-EP402222GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Adenylate kinase (adk) CSB-YP402223GEI
CSB-EP402223GEI
CSB-BP402223GEI
CSB-MP402223GEI
CSB-EP402223GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L1 (rplA) CSB-YP402224GEI
CSB-EP402224GEI
CSB-BP402224GEI
CSB-MP402224GEI
CSB-EP402224GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens DNA repair protein recO (recO) CSB-YP402225GEI
CSB-EP402225GEI
CSB-BP402225GEI
CSB-MP402225GEI
CSB-EP402225GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens UPF0234 protein Gura_0717 (Gura_0717) CSB-YP402226GEI
CSB-EP402226GEI
CSB-BP402226GEI
CSB-MP402226GEI
CSB-EP402226GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens NADH-quinone oxidoreductase subunit K 1 (nuoK1), partial CSB-YP402227GEI1
CSB-EP402227GEI1
CSB-BP402227GEI1
CSB-MP402227GEI1
CSB-EP402227GEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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