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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Mycoplasma agalactiae Uracil phosphoribosyltransferase (upp) CSB-YP402128MWC
CSB-EP402128MWC
CSB-BP402128MWC
CSB-MP402128MWC
CSB-EP402128MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP402129MWC
CSB-EP402129MWC
CSB-BP402129MWC
CSB-MP402129MWC
CSB-EP402129MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae GTPase obg (obg) CSB-YP402130MWC
CSB-EP402130MWC
CSB-BP402130MWC
CSB-MP402130MWC
CSB-EP402130MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 30S ribosomal protein S17 (rpsQ) CSB-YP402131MWC
CSB-EP402131MWC
CSB-BP402131MWC
CSB-MP402131MWC
CSB-EP402131MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L2 (rplB) CSB-YP402132MWC
CSB-EP402132MWC
CSB-BP402132MWC
CSB-MP402132MWC
CSB-EP402132MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 30S ribosomal protein S12 (rpsL) CSB-YP402133MWC
CSB-EP402133MWC
CSB-BP402133MWC
CSB-MP402133MWC
CSB-EP402133MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Tyrosine--tRNA ligase (tyrS) CSB-YP402134MWC
CSB-EP402134MWC
CSB-BP402134MWC
CSB-MP402134MWC
CSB-EP402134MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (PVX_091260), partial CSB-YP402135EWW
CSB-EP402135EWW
CSB-BP402135EWW
CSB-MP402135EWW
CSB-EP402135EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 30S ribosomal protein S16 (rpsP) CSB-YP402136FDT
CSB-EP402136FDT
CSB-BP402136FDT
CSB-MP402136FDT
CSB-EP402136FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L25 (rplY) CSB-YP402137FDT
CSB-EP402137FDT
CSB-BP402137FDT
CSB-MP402137FDT
CSB-EP402137FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Glutamate--tRNA ligase (gltX), partial CSB-YP402138FDT
CSB-EP402138FDT
CSB-BP402138FDT
CSB-MP402138FDT
CSB-EP402138FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Na (+)/H (+) antiporter nhaA 1, partial CSB-YP402139FDT
CSB-EP402139FDT
CSB-BP402139FDT
CSB-MP402139FDT
CSB-EP402139FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP402140FDT
CSB-EP402140FDT
CSB-BP402140FDT
CSB-MP402140FDT
CSB-EP402140FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP402141FDT
CSB-EP402141FDT
CSB-BP402141FDT
CSB-MP402141FDT
CSB-EP402141FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae UPF0758 protein Fjoh_0413 (Fjoh_0413) CSB-YP402142FDT
CSB-EP402142FDT
CSB-BP402142FDT
CSB-MP402142FDT
CSB-EP402142FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L2 (rplB) CSB-YP402143FDT
CSB-EP402143FDT
CSB-BP402143FDT
CSB-MP402143FDT
CSB-EP402143FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 30S ribosomal protein S11 (rpsK) CSB-YP402144FDT
CSB-EP402144FDT
CSB-BP402144FDT
CSB-MP402144FDT
CSB-EP402144FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP402145FDT
CSB-EP402145FDT
CSB-BP402145FDT
CSB-MP402145FDT
CSB-EP402145FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP402146FDT
CSB-EP402146FDT
CSB-BP402146FDT
CSB-MP402146FDT
CSB-EP402146FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 30S ribosomal protein S20 (rpsT) CSB-YP402147DHU
CSB-EP402147DHU
CSB-BP402147DHU
CSB-MP402147DHU
CSB-EP402147DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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