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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Adenylosuccinate synthetase (purA) CSB-YP402248SVC
CSB-EP402248SVC
CSB-BP402248SVC
CSB-MP402248SVC
CSB-EP402248SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP402249SVC
CSB-EP402249SVC
CSB-BP402249SVC
CSB-MP402249SVC
CSB-EP402249SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Gamma-glutamyl phosphate reductase (proA) CSB-YP402250SVC
CSB-EP402250SVC
CSB-BP402250SVC
CSB-MP402250SVC
CSB-EP402250SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP402251SVC
CSB-EP402251SVC
CSB-BP402251SVC
CSB-MP402251SVC
CSB-EP402251SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP402252SVC
CSB-EP402252SVC
CSB-BP402252SVC
CSB-MP402252SVC
CSB-EP402252SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP402253SVC
CSB-EP402253SVC
CSB-BP402253SVC
CSB-MP402253SVC
CSB-EP402253SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S18 (rpsR) CSB-YP402254SVC
CSB-EP402254SVC
CSB-BP402254SVC
CSB-MP402254SVC
CSB-EP402254SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP402255SVC
CSB-EP402255SVC
CSB-BP402255SVC
CSB-MP402255SVC
CSB-EP402255SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 2-isopropylmalate synthase (leuA), partial CSB-YP402256SVC
CSB-EP402256SVC
CSB-BP402256SVC
CSB-MP402256SVC
CSB-EP402256SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II D2 protein (psbD1), partial CSB-YP402257SVC1
CSB-EP402257SVC1
CSB-BP402257SVC1
CSB-MP402257SVC1
CSB-EP402257SVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA (guanine-N (1)-)-methyltransferase CSB-YP402258SVC
CSB-EP402258SVC
CSB-BP402258SVC
CSB-MP402258SVC
CSB-EP402258SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP402259SVC
CSB-EP402259SVC
CSB-BP402259SVC
CSB-MP402259SVC
CSB-EP402259SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP402260SVC
CSB-EP402260SVC
CSB-BP402260SVC
CSB-MP402260SVC
CSB-EP402260SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable cytosol aminopeptidase (pepA) CSB-YP402261SVC
CSB-EP402261SVC
CSB-BP402261SVC
CSB-MP402261SVC
CSB-EP402261SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribonuclease Z (rnz) CSB-YP402262SVC
CSB-EP402262SVC
CSB-BP402262SVC
CSB-MP402262SVC
CSB-EP402262SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP402263SVC
CSB-EP402263SVC
CSB-BP402263SVC
CSB-MP402263SVC
CSB-EP402263SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II 12 kDa extrinsic protein (psbU) CSB-YP402264SVC
CSB-EP402264SVC
CSB-BP402264SVC
CSB-MP402264SVC
CSB-EP402264SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit I CSB-YP402265SVC
CSB-EP402265SVC
CSB-BP402265SVC
CSB-MP402265SVC
CSB-EP402265SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Arginine--tRNA ligase (argS), partial CSB-YP402266SVC
CSB-EP402266SVC
CSB-BP402266SVC
CSB-MP402266SVC
CSB-EP402266SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP402267SVC
CSB-EP402267SVC
CSB-BP402267SVC
CSB-MP402267SVC
CSB-EP402267SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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