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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium kluyveri 50S ribosomal protein L23 (rplW) CSB-YP402488CWX
CSB-EP402488CWX
CSB-BP402488CWX
CSB-MP402488CWX
CSB-EP402488CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L29 (rpmC) CSB-YP402489CWX
CSB-EP402489CWX
CSB-BP402489CWX
CSB-MP402489CWX
CSB-EP402489CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S14 type Z (rpsZ) CSB-YP402490CWX
CSB-EP402490CWX
CSB-BP402490CWX
CSB-MP402490CWX
CSB-EP402490CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Translation initiation factor IF-1 (infA) CSB-YP402491CWX
CSB-EP402491CWX
CSB-BP402491CWX
CSB-MP402491CWX
CSB-EP402491CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Phosphoglucosamine mutase (glmM) CSB-YP402492CWX
CSB-EP402492CWX
CSB-BP402492CWX
CSB-MP402492CWX
CSB-EP402492CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 10 kDa chaperonin (groS) CSB-YP402493CWX
CSB-EP402493CWX
CSB-BP402493CWX
CSB-MP402493CWX
CSB-EP402493CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri UPF0229 protein CKL_0641 (CKL_0641) CSB-YP402494CWX
CSB-EP402494CWX
CSB-BP402494CWX
CSB-MP402494CWX
CSB-EP402494CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Quinolinate synthase A (nadA) CSB-YP402495CWX
CSB-EP402495CWX
CSB-BP402495CWX
CSB-MP402495CWX
CSB-EP402495CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP402496CWX
CSB-EP402496CWX
CSB-BP402496CWX
CSB-MP402496CWX
CSB-EP402496CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S21 (rpsU) CSB-YP402497CWX
CSB-EP402497CWX
CSB-BP402497CWX
CSB-MP402497CWX
CSB-EP402497CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP402498CWX
CSB-EP402498CWX
CSB-BP402498CWX
CSB-MP402498CWX
CSB-EP402498CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP402499CWX
CSB-EP402499CWX
CSB-BP402499CWX
CSB-MP402499CWX
CSB-EP402499CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Phosphopentomutase (deoB) CSB-YP402500CWX
CSB-EP402500CWX
CSB-BP402500CWX
CSB-MP402500CWX
CSB-EP402500CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP402501CWX
CSB-EP402501CWX
CSB-BP402501CWX
CSB-MP402501CWX
CSB-EP402501CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri GTPase Der (der) CSB-YP402502CWX
CSB-EP402502CWX
CSB-BP402502CWX
CSB-MP402502CWX
CSB-EP402502CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Acetate kinase (ackA) CSB-YP402503CWX
CSB-EP402503CWX
CSB-BP402503CWX
CSB-MP402503CWX
CSB-EP402503CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 50S ribosomal protein L19 (rplS) CSB-YP402504CWX
CSB-EP402504CWX
CSB-BP402504CWX
CSB-MP402504CWX
CSB-EP402504CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S2 (rpsB) CSB-YP402505CWX
CSB-EP402505CWX
CSB-BP402505CWX
CSB-MP402505CWX
CSB-EP402505CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Ribonuclease Y (rny), partial CSB-YP402506CWX
CSB-EP402506CWX
CSB-BP402506CWX
CSB-MP402506CWX
CSB-EP402506CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Protein hfq (hfq) CSB-YP402507CWX
CSB-EP402507CWX
CSB-BP402507CWX
CSB-MP402507CWX
CSB-EP402507CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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