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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium kluyveri Adenylyl-sulfate kinase (cysC) CSB-YP402508CWX
CSB-EP402508CWX
CSB-BP402508CWX
CSB-MP402508CWX
CSB-EP402508CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Plasma cell-induced resident endoplasmic reticulum protein (PACAP) CSB-YP402509BO
CSB-EP402509BO
CSB-BP402509BO
CSB-MP402509BO
CSB-EP402509BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein CXorf64 homolog CSB-YP402510BO
CSB-EP402510BO
CSB-BP402510BO
CSB-MP402510BO
CSB-EP402510BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein CXorf49 homolog, partial CSB-YP402511BO
CSB-EP402511BO
CSB-BP402511BO
CSB-MP402511BO
CSB-EP402511BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Ribosome maturation factor RimM (rimM) CSB-YP402512FDT
CSB-EP402512FDT
CSB-BP402512FDT
CSB-MP402512FDT
CSB-EP402512FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Glutamyl-tRNA reductase 1 (hemA1) CSB-YP402513FDT
CSB-EP402513FDT
CSB-BP402513FDT
CSB-MP402513FDT
CSB-EP402513FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP402514FDT
CSB-EP402514FDT
CSB-BP402514FDT
CSB-MP402514FDT
CSB-EP402514FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Leucine--tRNA ligase (leuS), partial CSB-YP402515FDT
CSB-EP402515FDT
CSB-BP402515FDT
CSB-MP402515FDT
CSB-EP402515FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae D-tyrosyl-tRNA (Tyr) deacylase CSB-YP402516FDT
CSB-EP402516FDT
CSB-BP402516FDT
CSB-MP402516FDT
CSB-EP402516FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae GTPase obg (obg) CSB-YP402517FDT
CSB-EP402517FDT
CSB-BP402517FDT
CSB-MP402517FDT
CSB-EP402517FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L29 (rpmC) CSB-YP402518FDT
CSB-EP402518FDT
CSB-BP402518FDT
CSB-MP402518FDT
CSB-EP402518FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Translation initiation factor IF-1 (infA) CSB-YP402519FDT
CSB-EP402519FDT
CSB-BP402519FDT
CSB-MP402519FDT
CSB-EP402519FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L17 (rplQ) CSB-YP402520FDT
CSB-EP402520FDT
CSB-BP402520FDT
CSB-MP402520FDT
CSB-EP402520FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Bifunctional protein FolD (folD) CSB-YP402521FDT
CSB-EP402521FDT
CSB-BP402521FDT
CSB-MP402521FDT
CSB-EP402521FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae DNA replication and repair protein recF (recF) CSB-YP402522FDT
CSB-EP402522FDT
CSB-BP402522FDT
CSB-MP402522FDT
CSB-EP402522FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L20 (rplT) CSB-YP402523FDT
CSB-EP402523FDT
CSB-BP402523FDT
CSB-MP402523FDT
CSB-EP402523FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP402524DHU
CSB-EP402524DHU
CSB-BP402524DHU
CSB-MP402524DHU
CSB-EP402524DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP402525DHU
CSB-EP402525DHU
CSB-BP402525DHU
CSB-MP402525DHU
CSB-EP402525DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Tryptophan synthase alpha chain (trpA) CSB-YP402526DHU
CSB-EP402526DHU
CSB-BP402526DHU
CSB-MP402526DHU
CSB-EP402526DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Heat-inducible transcription repressor HrcA (hrcA) CSB-YP402527DHU
CSB-EP402527DHU
CSB-BP402527DHU
CSB-MP402527DHU
CSB-EP402527DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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