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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma agalactiae 30S ribosomal protein S16 (rpsP) CSB-YP402468MWC
CSB-EP402468MWC
CSB-BP402468MWC
CSB-MP402468MWC
CSB-EP402468MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax Lipoyl synthase, apicoplast (lipA) CSB-YP402469EWW
CSB-EP402469EWW
CSB-BP402469EWW
CSB-MP402469EWW
CSB-EP402469EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Uncharacterized protein C19orf71 homolog (QtsA-14935) CSB-YP402470MOV
CSB-EP402470MOV
CSB-BP402470MOV
CSB-MP402470MOV
CSB-EP402470MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Carbon storage regulator homolog (csrA) CSB-YP402471CWX
CSB-EP402471CWX
CSB-BP402471CWX
CSB-MP402471CWX
CSB-EP402471CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 2-isopropylmalate synthase (leuA), partial CSB-YP402472CWX
CSB-EP402472CWX
CSB-BP402472CWX
CSB-MP402472CWX
CSB-EP402472CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Gamma-glutamyl phosphate reductase (proA) CSB-YP402473CWX
CSB-EP402473CWX
CSB-BP402473CWX
CSB-MP402473CWX
CSB-EP402473CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Histidine--tRNA ligase (hisS) CSB-YP402474CWX
CSB-EP402474CWX
CSB-BP402474CWX
CSB-MP402474CWX
CSB-EP402474CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Anti-sigma F factor (spoIIAB) CSB-YP402475CWX
CSB-EP402475CWX
CSB-BP402475CWX
CSB-MP402475CWX
CSB-EP402475CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP402476CWX
CSB-EP402476CWX
CSB-BP402476CWX
CSB-MP402476CWX
CSB-EP402476CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP402477CWX
CSB-EP402477CWX
CSB-BP402477CWX
CSB-MP402477CWX
CSB-EP402477CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri DNA polymerase IV (dinB) CSB-YP402478CWX
CSB-EP402478CWX
CSB-BP402478CWX
CSB-MP402478CWX
CSB-EP402478CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Glycogen synthase (glgA) CSB-YP402479CWX
CSB-EP402479CWX
CSB-BP402479CWX
CSB-MP402479CWX
CSB-EP402479CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Protein translocase subunit SecA (secA), partial CSB-YP402480CWX
CSB-EP402480CWX
CSB-BP402480CWX
CSB-MP402480CWX
CSB-EP402480CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Uracil phosphoribosyltransferase (upp) CSB-YP402481CWX
CSB-EP402481CWX
CSB-BP402481CWX
CSB-MP402481CWX
CSB-EP402481CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S6 (rpsF) CSB-YP402482CWX
CSB-EP402482CWX
CSB-BP402482CWX
CSB-MP402482CWX
CSB-EP402482CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri DNA replication and repair protein recF (recF) CSB-YP402483CWX
CSB-EP402483CWX
CSB-BP402483CWX
CSB-MP402483CWX
CSB-EP402483CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Adenylosuccinate synthetase (purA) CSB-YP402484CWX
CSB-EP402484CWX
CSB-BP402484CWX
CSB-MP402484CWX
CSB-EP402484CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Peptidyl-tRNA hydrolase (pth) CSB-YP402485CWX
CSB-EP402485CWX
CSB-BP402485CWX
CSB-MP402485CWX
CSB-EP402485CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Glycine--tRNA ligase (glyQS) CSB-YP402486CWX
CSB-EP402486CWX
CSB-BP402486CWX
CSB-MP402486CWX
CSB-EP402486CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri 30S ribosomal protein S12 (rpsL) CSB-YP402487CWX
CSB-EP402487CWX
CSB-BP402487CWX
CSB-MP402487CWX
CSB-EP402487CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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