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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter uraniireducens Gamma-glutamyl phosphate reductase (proA) CSB-YP402588GEI
CSB-EP402588GEI
CSB-BP402588GEI
CSB-MP402588GEI
CSB-EP402588GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Cysteine--tRNA ligase (cysS) CSB-YP402589GEI
CSB-EP402589GEI
CSB-BP402589GEI
CSB-MP402589GEI
CSB-EP402589GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens NADH-quinone oxidoreductase subunit A 2 (nuoA2), partial CSB-YP402590GEI1
CSB-EP402590GEI1
CSB-BP402590GEI1
CSB-MP402590GEI1
CSB-EP402590GEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Histidinol-phosphate aminotransferase (hisC) CSB-YP402591GEI
CSB-EP402591GEI
CSB-BP402591GEI
CSB-MP402591GEI
CSB-EP402591GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Probable potassium transport system protein kup (kup), partial CSB-YP402592GEI
CSB-EP402592GEI
CSB-BP402592GEI
CSB-MP402592GEI
CSB-EP402592GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Putative membrane protein insertion efficiency factor (Gura_4430) CSB-YP402593GEI
CSB-EP402593GEI
CSB-BP402593GEI
CSB-MP402593GEI
CSB-EP402593GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens S-adenosylmethionine synthase (metK) CSB-YP402594GEI
CSB-EP402594GEI
CSB-BP402594GEI
CSB-MP402594GEI
CSB-EP402594GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens LexA repressor (lexA) CSB-YP402595GEI
CSB-EP402595GEI
CSB-BP402595GEI
CSB-MP402595GEI
CSB-EP402595GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 30S ribosomal protein S8 (rpsH) CSB-YP402596GEI
CSB-EP402596GEI
CSB-BP402596GEI
CSB-MP402596GEI
CSB-EP402596GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 50S ribosomal protein L14 (rplN) CSB-YP402597GEI
CSB-EP402597GEI
CSB-BP402597GEI
CSB-MP402597GEI
CSB-EP402597GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP402598GEI
CSB-EP402598GEI
CSB-BP402598GEI
CSB-MP402598GEI
CSB-EP402598GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP402599GEI
CSB-EP402599GEI
CSB-BP402599GEI
CSB-MP402599GEI
CSB-EP402599GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Dihydrodipicolinate synthase (dapA) CSB-YP402600GEI
CSB-EP402600GEI
CSB-BP402600GEI
CSB-MP402600GEI
CSB-EP402600GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP402601GEI
CSB-EP402601GEI
CSB-BP402601GEI
CSB-MP402601GEI
CSB-EP402601GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP402602GEI
CSB-EP402602GEI
CSB-BP402602GEI
CSB-MP402602GEI
CSB-EP402602GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens tRNA dimethylallyltransferase 2 (miaA2) CSB-YP402603GEI
CSB-EP402603GEI
CSB-BP402603GEI
CSB-MP402603GEI
CSB-EP402603GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Dihydroorotase (pyrC) CSB-YP402604GEI
CSB-EP402604GEI
CSB-BP402604GEI
CSB-MP402604GEI
CSB-EP402604GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter uraniireducens Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP402605GEI
CSB-EP402605GEI
CSB-BP402605GEI
CSB-MP402605GEI
CSB-EP402605GEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Trigger factor (tig) CSB-YP402606SVC
CSB-EP402606SVC
CSB-BP402606SVC
CSB-MP402606SVC
CSB-EP402606SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Histidine--tRNA ligase (hisS) CSB-YP402607SVC
CSB-EP402607SVC
CSB-BP402607SVC
CSB-MP402607SVC
CSB-EP402607SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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