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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum Cobyric acid synthase (cobQ) CSB-YP402668CWV
CSB-EP402668CWV
CSB-BP402668CWV
CSB-MP402668CWV
CSB-EP402668CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate--tRNA ligase (gltX) CSB-YP402669CWV
CSB-EP402669CWV
CSB-BP402669CWV
CSB-MP402669CWV
CSB-EP402669CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative 3-methyladenine DNA glycosylase (CBO1265, CLC_1303) CSB-YP402670CWV
CSB-EP402670CWV
CSB-BP402670CWV
CSB-MP402670CWV
CSB-EP402670CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Cell division protein DivIB (divIB), partial CSB-YP402671CWV
CSB-EP402671CWV
CSB-BP402671CWV
CSB-MP402671CWV
CSB-EP402671CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP402672CWV
CSB-EP402672CWV
CSB-BP402672CWV
CSB-MP402672CWV
CSB-EP402672CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Arginine repressor (argR) CSB-YP402673CWV
CSB-EP402673CWV
CSB-BP402673CWV
CSB-MP402673CWV
CSB-EP402673CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0597 protein CBO1964/CLC_1909 (CBO1964, CLC_1909) CSB-YP402674CWV
CSB-EP402674CWV
CSB-BP402674CWV
CSB-MP402674CWV
CSB-EP402674CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Biotin synthase (bioB) CSB-YP402675CWV
CSB-EP402675CWV
CSB-BP402675CWV
CSB-MP402675CWV
CSB-EP402675CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome maturation factor RimP (rimP) CSB-YP402676CWV
CSB-EP402676CWV
CSB-BP402676CWV
CSB-MP402676CWV
CSB-EP402676CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0122 protein CBO2450/CLC_2298 (CBO2450, CLC_2298) CSB-YP402677CWV
CSB-EP402677CWV
CSB-BP402677CWV
CSB-MP402677CWV
CSB-EP402677CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0296 protein CBO2514/CLC_2370 (CBO2514, CLC_2370) CSB-YP402678CWV
CSB-EP402678CWV
CSB-BP402678CWV
CSB-MP402678CWV
CSB-EP402678CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Alanine--tRNA ligase (alaS), partial CSB-YP402679CWV
CSB-EP402679CWV
CSB-BP402679CWV
CSB-MP402679CWV
CSB-EP402679CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glycerol kinase (glpK) CSB-YP402680CWV
CSB-EP402680CWV
CSB-BP402680CWV
CSB-MP402680CWV
CSB-EP402680CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP402681CWV
CSB-EP402681CWV
CSB-BP402681CWV
CSB-MP402681CWV
CSB-EP402681CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal protein L11 methyltransferase (prmA) CSB-YP402682CWV
CSB-EP402682CWV
CSB-BP402682CWV
CSB-MP402682CWV
CSB-EP402682CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase obg (obg) CSB-YP402683CWV
CSB-EP402683CWV
CSB-BP402683CWV
CSB-MP402683CWV
CSB-EP402683CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0735 protein CBO3010/CLC_2906 (CBO3010, CLC_2906) CSB-YP402684CWV
CSB-EP402684CWV
CSB-BP402684CWV
CSB-MP402684CWV
CSB-EP402684CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP402685CWV
CSB-EP402685CWV
CSB-BP402685CWV
CSB-MP402685CWV
CSB-EP402685CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Threonine--tRNA ligase (thrS), partial CSB-YP402686CWV
CSB-EP402686CWV
CSB-BP402686CWV
CSB-MP402686CWV
CSB-EP402686CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP402687CWV
CSB-EP402687CWV
CSB-BP402687CWV
CSB-MP402687CWV
CSB-EP402687CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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