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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila 50S ribosomal protein L10 (rplJ) CSB-YP402728LLJ
CSB-EP402728LLJ
CSB-BP402728LLJ
CSB-MP402728LLJ
CSB-EP402728LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribosome-binding factor A (rbfA) CSB-YP402729LLJ
CSB-EP402729LLJ
CSB-BP402729LLJ
CSB-MP402729LLJ
CSB-EP402729LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP402730LLJ
CSB-EP402730LLJ
CSB-BP402730LLJ
CSB-MP402730LLJ
CSB-EP402730LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila NAD (P)H-hydrate epimerase CSB-YP402731LLJ
CSB-EP402731LLJ
CSB-BP402731LLJ
CSB-MP402731LLJ
CSB-EP402731LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP402732LLJ1
CSB-EP402732LLJ1
CSB-BP402732LLJ1
CSB-MP402732LLJ1
CSB-EP402732LLJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP402733LLJ
CSB-EP402733LLJ
CSB-BP402733LLJ
CSB-MP402733LLJ
CSB-EP402733LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP402734LLJ
CSB-EP402734LLJ
CSB-BP402734LLJ
CSB-MP402734LLJ
CSB-EP402734LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila UPF0102 protein LPC_3309 (LPC_3309) CSB-YP402735LLJ
CSB-EP402735LLJ
CSB-BP402735LLJ
CSB-MP402735LLJ
CSB-EP402735LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Homoserine O-succinyltransferase (metA) CSB-YP402736TKF
CSB-EP402736TKF
CSB-BP402736TKF
CSB-MP402736TKF
CSB-EP402736TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Heat-inducible transcription repressor HrcA (hrcA) CSB-YP402737TKF
CSB-EP402737TKF
CSB-BP402737TKF
CSB-MP402737TKF
CSB-EP402737TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Maritimacin (Tpet_0144) CSB-YP402738TKF
CSB-EP402738TKF
CSB-BP402738TKF
CSB-MP402738TKF
CSB-EP402738TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP402739TKF
CSB-EP402739TKF
CSB-BP402739TKF
CSB-MP402739TKF
CSB-EP402739TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Phosphoglycerate kinase (pgk) CSB-YP402740TKF
CSB-EP402740TKF
CSB-BP402740TKF
CSB-MP402740TKF
CSB-EP402740TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP402741TKF
CSB-EP402741TKF
CSB-BP402741TKF
CSB-MP402741TKF
CSB-EP402741TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP402742TKF
CSB-EP402742TKF
CSB-BP402742TKF
CSB-MP402742TKF
CSB-EP402742TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Chaperone protein DnaK (dnaK), partial CSB-YP402743TKF
CSB-EP402743TKF
CSB-BP402743TKF
CSB-MP402743TKF
CSB-EP402743TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila UPF0102 protein Tpet_0671 (Tpet_0671) CSB-YP402744TKF
CSB-EP402744TKF
CSB-BP402744TKF
CSB-MP402744TKF
CSB-EP402744TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP402745TKF
CSB-EP402745TKF
CSB-BP402745TKF
CSB-MP402745TKF
CSB-EP402745TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila DNA ligase (ligA), partial CSB-YP402746TKF
CSB-EP402746TKF
CSB-BP402746TKF
CSB-MP402746TKF
CSB-EP402746TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Nucleoside-triphosphatase THEP1 (Tpet_0887) CSB-YP402747TKF
CSB-EP402747TKF
CSB-BP402747TKF
CSB-MP402747TKF
CSB-EP402747TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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