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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanobrevibacter smithii 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP403028MNN
CSB-EP403028MNN
CSB-BP403028MNN
CSB-MP403028MNN
CSB-EP403028MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP403029MNN
CSB-EP403029MNN
CSB-BP403029MNN
CSB-MP403029MNN
CSB-EP403029MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii UPF0103 protein Msm_1438 (Msm_1438) CSB-YP403030MNN
CSB-EP403030MNN
CSB-BP403030MNN
CSB-MP403030MNN
CSB-EP403030MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP403031MNN
CSB-EP403031MNN
CSB-BP403031MNN
CSB-MP403031MNN
CSB-EP403031MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Prefoldin subunit beta (pfdB) CSB-YP403032MNN
CSB-EP403032MNN
CSB-BP403032MNN
CSB-MP403032MNN
CSB-EP403032MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Transcription elongation factor GreA (greA) CSB-YP403033RIQ
CSB-EP403033RIQ
CSB-BP403033RIQ
CSB-MP403033RIQ
CSB-EP403033RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 50S ribosomal protein L25 (rplY) CSB-YP403034RIQ
CSB-EP403034RIQ
CSB-BP403034RIQ
CSB-MP403034RIQ
CSB-EP403034RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. ATP synthase gamma chain (atpG) CSB-YP403035RIQ
CSB-EP403035RIQ
CSB-BP403035RIQ
CSB-MP403035RIQ
CSB-EP403035RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP403036RIQ
CSB-EP403036RIQ
CSB-BP403036RIQ
CSB-MP403036RIQ
CSB-EP403036RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP403037RIQ
CSB-EP403037RIQ
CSB-BP403037RIQ
CSB-MP403037RIQ
CSB-EP403037RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S15 (rpsO) CSB-YP403038RIQ
CSB-EP403038RIQ
CSB-BP403038RIQ
CSB-MP403038RIQ
CSB-EP403038RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP403039RIQ
CSB-EP403039RIQ
CSB-BP403039RIQ
CSB-MP403039RIQ
CSB-EP403039RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S4 (rpsD) CSB-YP403040RIQ
CSB-EP403040RIQ
CSB-BP403040RIQ
CSB-MP403040RIQ
CSB-EP403040RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S5 (rpsE) CSB-YP403041RIQ
CSB-EP403041RIQ
CSB-BP403041RIQ
CSB-MP403041RIQ
CSB-EP403041RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 50S ribosomal protein L24 (rplX) CSB-YP403042RIQ
CSB-EP403042RIQ
CSB-BP403042RIQ
CSB-MP403042RIQ
CSB-EP403042RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. 30S ribosomal protein S3 (rpsC) CSB-YP403043RIQ
CSB-EP403043RIQ
CSB-BP403043RIQ
CSB-MP403043RIQ
CSB-EP403043RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP403044RIQ
CSB-EP403044RIQ
CSB-BP403044RIQ
CSB-MP403044RIQ
CSB-EP403044RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Glutamate--tRNA ligase (gltX), partial CSB-YP403045RIQ
CSB-EP403045RIQ
CSB-BP403045RIQ
CSB-MP403045RIQ
CSB-EP403045RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP403046RIQ
CSB-EP403046RIQ
CSB-BP403046RIQ
CSB-MP403046RIQ
CSB-EP403046RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus sp. Uridylate kinase (pyrH) CSB-YP403047RIQ
CSB-EP403047RIQ
CSB-BP403047RIQ
CSB-MP403047RIQ
CSB-EP403047RIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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