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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haemophilus influenzae Chaperone protein DnaJ (dnaJ) CSB-YP402968HSE
CSB-EP402968HSE
CSB-BP402968HSE
CSB-MP402968HSE
CSB-EP402968HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP402969HSE
CSB-EP402969HSE
CSB-BP402969HSE
CSB-MP402969HSE
CSB-EP402969HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP402970HSE
CSB-EP402970HSE
CSB-BP402970HSE
CSB-MP402970HSE
CSB-EP402970HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae UPF0271 protein CGSHiGG_02380 (CGSHiGG_02380) CSB-YP402971HSE
CSB-EP402971HSE
CSB-BP402971HSE
CSB-MP402971HSE
CSB-EP402971HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Protein FdhE homolog (fdhE) CSB-YP402972HSE
CSB-EP402972HSE
CSB-BP402972HSE
CSB-MP402972HSE
CSB-EP402972HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Citrate lyase acyl carrier protein (citD) CSB-YP402973HSE
CSB-EP402973HSE
CSB-BP402973HSE
CSB-MP402973HSE
CSB-EP402973HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Mannonate dehydratase (uxuA) CSB-YP402974HSE
CSB-EP402974HSE
CSB-BP402974HSE
CSB-MP402974HSE
CSB-EP402974HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Uridine kinase (udk) CSB-YP402975HSE
CSB-EP402975HSE
CSB-BP402975HSE
CSB-MP402975HSE
CSB-EP402975HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae N-acetylneuraminate epimerase (nanM) CSB-YP402976HSE
CSB-EP402976HSE
CSB-BP402976HSE
CSB-MP402976HSE
CSB-EP402976HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Na (+)-translocating NADH-quinone reductase subunit D, partial CSB-YP402977HSE1
CSB-EP402977HSE1
CSB-BP402977HSE1
CSB-MP402977HSE1
CSB-EP402977HSE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Shikimate kinase (aroK) CSB-YP402978HSE
CSB-EP402978HSE
CSB-BP402978HSE
CSB-MP402978HSE
CSB-EP402978HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP402979HSE
CSB-EP402979HSE
CSB-BP402979HSE
CSB-MP402979HSE
CSB-EP402979HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae UPF0225 protein CGSHiGG_04185 (CGSHiGG_04185) CSB-YP402980HSE
CSB-EP402980HSE
CSB-BP402980HSE
CSB-MP402980HSE
CSB-EP402980HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Adenylate kinase (adk) CSB-YP402981HSE
CSB-EP402981HSE
CSB-BP402981HSE
CSB-MP402981HSE
CSB-EP402981HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Protein RnfH (rnfH) CSB-YP402982HSE
CSB-EP402982HSE
CSB-BP402982HSE
CSB-MP402982HSE
CSB-EP402982HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Fe/S biogenesis protein NfuA (nfuA) CSB-YP402983HSE
CSB-EP402983HSE
CSB-BP402983HSE
CSB-MP402983HSE
CSB-EP402983HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Bifunctional protein pyrR (pyrR) CSB-YP402984HSE
CSB-EP402984HSE
CSB-BP402984HSE
CSB-MP402984HSE
CSB-EP402984HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae ATP synthase gamma chain (atpG) CSB-YP402985HSE
CSB-EP402985HSE
CSB-BP402985HSE
CSB-MP402985HSE
CSB-EP402985HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Regulator of ribonuclease activity A (rraA) CSB-YP402986HSE
CSB-EP402986HSE
CSB-BP402986HSE
CSB-MP402986HSE
CSB-EP402986HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Urease accessory protein UreF (ureF) CSB-YP402987HSE
CSB-EP402987HSE
CSB-BP402987HSE
CSB-MP402987HSE
CSB-EP402987HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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