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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella ovis Probable transaldolase (tal) CSB-YP403950BPI
CSB-EP403950BPI
CSB-BP403950BPI
CSB-MP403950BPI
CSB-EP403950BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Gamma-glutamyl phosphate reductase (proA) CSB-YP403951BPI
CSB-EP403951BPI
CSB-BP403951BPI
CSB-MP403951BPI
CSB-EP403951BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis tRNA modification GTPase MnmE (mnmE) CSB-YP403952BPI
CSB-EP403952BPI
CSB-BP403952BPI
CSB-MP403952BPI
CSB-EP403952BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Shikimate dehydrogenase (aroE) CSB-YP403953BPI
CSB-EP403953BPI
CSB-BP403953BPI
CSB-MP403953BPI
CSB-EP403953BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP403954BPI
CSB-EP403954BPI
CSB-BP403954BPI
CSB-MP403954BPI
CSB-EP403954BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP403955BPI
CSB-EP403955BPI
CSB-BP403955BPI
CSB-MP403955BPI
CSB-EP403955BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP403956BPI
CSB-EP403956BPI
CSB-BP403956BPI
CSB-MP403956BPI
CSB-EP403956BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Flagellar hook-basal body complex protein FliE (fliE) CSB-YP403957BPI
CSB-EP403957BPI
CSB-BP403957BPI
CSB-MP403957BPI
CSB-EP403957BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Putative peptide permease protein BOV_A0350 (BOV_A0350), partial CSB-YP403958BPI1
CSB-EP403958BPI1
CSB-BP403958BPI1
CSB-MP403958BPI1
CSB-EP403958BPI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Biotin synthase (bioB) CSB-YP403959BPI
CSB-EP403959BPI
CSB-BP403959BPI
CSB-MP403959BPI
CSB-EP403959BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP403960BPI
CSB-EP403960BPI
CSB-BP403960BPI
CSB-MP403960BPI
CSB-EP403960BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cysteine-rich and transmembrane domain-containing protein 1 (cystm1), partial CSB-YP403961DIL1
CSB-EP403961DIL1
CSB-BP403961DIL1
CSB-MP403961DIL1
CSB-EP403961DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (lhpp) CSB-YP403962DIL
CSB-EP403962DIL
CSB-BP403962DIL
CSB-MP403962DIL
CSB-EP403962DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis UPF0301 protein MRA_0041 (MRA_0041) CSB-YP403963MON
CSB-EP403963MON
CSB-BP403963MON
CSB-MP403963MON
CSB-EP403963MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Phosphoheptose isomerase (gmhA) CSB-YP403964MON
CSB-EP403964MON
CSB-BP403964MON
CSB-MP403964MON
CSB-EP403964MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis R2-like ligand binding oxidase (MRA_0241) CSB-YP403965MON
CSB-EP403965MON
CSB-BP403965MON
CSB-MP403965MON
CSB-EP403965MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Chaperone protein DnaK (dnaK), partial CSB-YP403966MON
CSB-EP403966MON
CSB-BP403966MON
CSB-MP403966MON
CSB-EP403966MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Carboxylate-amine ligase MRA_0438 (MRA_0438) CSB-YP403967MON
CSB-EP403967MON
CSB-BP403967MON
CSB-MP403967MON
CSB-EP403967MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP403968MON
CSB-EP403968MON
CSB-BP403968MON
CSB-MP403968MON
CSB-EP403968MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP403969MON
CSB-EP403969MON
CSB-BP403969MON
CSB-MP403969MON
CSB-EP403969MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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