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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus reuteri Chaperone protein DnaJ (dnaJ) CSB-YP405010LLF
CSB-EP405010LLF
CSB-BP405010LLF
CSB-MP405010LLF
CSB-EP405010LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri D-tyrosyl-tRNA (Tyr) deacylase CSB-YP405011LLF
CSB-EP405011LLF
CSB-BP405011LLF
CSB-MP405011LLF
CSB-EP405011LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA repair protein recO (recO) CSB-YP405012LLF
CSB-EP405012LLF
CSB-BP405012LLF
CSB-MP405012LLF
CSB-EP405012LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Holliday junction resolvase recU (recU) CSB-YP405013LLF
CSB-EP405013LLF
CSB-BP405013LLF
CSB-MP405013LLF
CSB-EP405013LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP405014LLF
CSB-EP405014LLF
CSB-BP405014LLF
CSB-MP405014LLF
CSB-EP405014LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S14 (rpsN) CSB-YP405015LLF
CSB-EP405015LLF
CSB-BP405015LLF
CSB-MP405015LLF
CSB-EP405015LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Bifunctional protein FolD (folD) CSB-YP405016LLF
CSB-EP405016LLF
CSB-BP405016LLF
CSB-MP405016LLF
CSB-EP405016LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP405017LLF
CSB-EP405017LLF
CSB-BP405017LLF
CSB-MP405017LLF
CSB-EP405017LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L35 (rpmI) CSB-YP405018LLF
CSB-EP405018LLF
CSB-BP405018LLF
CSB-MP405018LLF
CSB-EP405018LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri UPF0342 protein Lreu_1268 (Lreu_1268) CSB-YP405019LLF
CSB-EP405019LLF
CSB-BP405019LLF
CSB-MP405019LLF
CSB-EP405019LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Regulatory protein recX (recX) CSB-YP405020LLF
CSB-EP405020LLF
CSB-BP405020LLF
CSB-MP405020LLF
CSB-EP405020LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L15 (rplO) CSB-YP405021LLF
CSB-EP405021LLF
CSB-BP405021LLF
CSB-MP405021LLF
CSB-EP405021LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S14 type Z (rpsZ) CSB-YP405022LLF
CSB-EP405022LLF
CSB-BP405022LLF
CSB-MP405022LLF
CSB-EP405022LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L22 (rplV) CSB-YP405023LLF
CSB-EP405023LLF
CSB-BP405023LLF
CSB-MP405023LLF
CSB-EP405023LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP405024LLF
CSB-EP405024LLF
CSB-BP405024LLF
CSB-MP405024LLF
CSB-EP405024LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri S-ribosylhomocysteine lyase (luxS) CSB-YP405025LLF
CSB-EP405025LLF
CSB-BP405025LLF
CSB-MP405025LLF
CSB-EP405025LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ferredoxin--NADP reductase (Lreu_1657) CSB-YP405026LLF
CSB-EP405026LLF
CSB-BP405026LLF
CSB-MP405026LLF
CSB-EP405026LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP405027LLF
CSB-EP405027LLF
CSB-BP405027LLF
CSB-MP405027LLF
CSB-EP405027LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Aspartate--ammonia ligase (asnA) CSB-YP405028LLF
CSB-EP405028LLF
CSB-BP405028LLF
CSB-MP405028LLF
CSB-EP405028LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Cytidylate kinase (cmk) CSB-YP405029BPI
CSB-EP405029BPI
CSB-BP405029BPI
CSB-MP405029BPI
CSB-EP405029BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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