Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella ovis Malate synthase G (glcB), partial CSB-YP405050BPI
CSB-EP405050BPI
CSB-BP405050BPI
CSB-MP405050BPI
CSB-EP405050BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP405051BPI
CSB-EP405051BPI
CSB-BP405051BPI
CSB-MP405051BPI
CSB-EP405051BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L36 (rpmJ) CSB-YP405052BPI
CSB-EP405052BPI
CSB-BP405052BPI
CSB-MP405052BPI
CSB-EP405052BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP405053BPI
CSB-EP405053BPI
CSB-BP405053BPI
CSB-MP405053BPI
CSB-EP405053BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP405054BPI
CSB-EP405054BPI
CSB-BP405054BPI
CSB-MP405054BPI
CSB-EP405054BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L20 (rplT) CSB-YP405055BPI
CSB-EP405055BPI
CSB-BP405055BPI
CSB-MP405055BPI
CSB-EP405055BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP405056BPI
CSB-EP405056BPI
CSB-BP405056BPI
CSB-MP405056BPI
CSB-EP405056BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Histidine--tRNA ligase (hisS), partial CSB-YP405057BPI
CSB-EP405057BPI
CSB-BP405057BPI
CSB-MP405057BPI
CSB-EP405057BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP405058BPI
CSB-EP405058BPI
CSB-BP405058BPI
CSB-MP405058BPI
CSB-EP405058BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis DNA mismatch repair protein mutL (mutL), partial CSB-YP405059BPI
CSB-EP405059BPI
CSB-BP405059BPI
CSB-MP405059BPI
CSB-EP405059BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis UPF0173 metal-dependent hydrolase BOV_A0561 (BOV_A0561) CSB-YP405060BPI
CSB-EP405060BPI
CSB-BP405060BPI
CSB-MP405060BPI
CSB-EP405060BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Lectin-like protein BA14k (BOV_A0688), partial CSB-YP405061BPI1
CSB-EP405061BPI1
CSB-BP405061BPI1
CSB-MP405061BPI1
CSB-EP405061BPI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis UPF0309 protein BOV_A0853 (BOV_A0853) CSB-YP405062BPI
CSB-EP405062BPI
CSB-BP405062BPI
CSB-MP405062BPI
CSB-EP405062BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L34 (rpmH) CSB-YP405063BPI
CSB-EP405063BPI
CSB-BP405063BPI
CSB-MP405063BPI
CSB-EP405063BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Methylglyoxal synthase (mgsA) CSB-YP405064BPI
CSB-EP405064BPI
CSB-BP405064BPI
CSB-MP405064BPI
CSB-EP405064BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP405065PZN
CSB-EP405065PZN
CSB-BP405065PZN
CSB-MP405065PZN
CSB-EP405065PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP405066PZN
CSB-EP405066PZN
CSB-BP405066PZN
CSB-MP405066PZN
CSB-EP405066PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 8-amino-7-oxononanoate synthase (bioF) CSB-YP405067PZN
CSB-EP405067PZN
CSB-BP405067PZN
CSB-MP405067PZN
CSB-EP405067PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP405068PZN
CSB-EP405068PZN
CSB-BP405068PZN
CSB-MP405068PZN
CSB-EP405068PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP405069PZN
CSB-EP405069PZN
CSB-BP405069PZN
CSB-MP405069PZN
CSB-EP405069PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>