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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Serine/threonine transporter sstT (sstT), partial CSB-YP405090PZN
CSB-EP405090PZN
CSB-BP405090PZN
CSB-MP405090PZN
CSB-EP405090PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP405091PZN
CSB-EP405091PZN
CSB-BP405091PZN
CSB-MP405091PZN
CSB-EP405091PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphate acyltransferase (plsX) CSB-YP405092PZN
CSB-EP405092PZN
CSB-BP405092PZN
CSB-MP405092PZN
CSB-EP405092PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0434 protein Pput_3813 (Pput_3813) CSB-YP405093PZN
CSB-EP405093PZN
CSB-BP405093PZN
CSB-MP405093PZN
CSB-EP405093PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP405094PZN
CSB-EP405094PZN
CSB-BP405094PZN
CSB-MP405094PZN
CSB-EP405094PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein sprT (sprT) CSB-YP405095PZN
CSB-EP405095PZN
CSB-BP405095PZN
CSB-MP405095PZN
CSB-EP405095PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP405096PZN
CSB-EP405096PZN
CSB-BP405096PZN
CSB-MP405096PZN
CSB-EP405096PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP405097PZN
CSB-EP405097PZN
CSB-BP405097PZN
CSB-MP405097PZN
CSB-EP405097PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP405098PZN
CSB-EP405098PZN
CSB-BP405098PZN
CSB-MP405098PZN
CSB-EP405098PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP405099PZN
CSB-EP405099PZN
CSB-BP405099PZN
CSB-MP405099PZN
CSB-EP405099PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA repair protein recO (recO) CSB-YP405100PZN
CSB-EP405100PZN
CSB-BP405100PZN
CSB-MP405100PZN
CSB-EP405100PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein translocase subunit SecA (secA), partial CSB-YP405101PZN
CSB-EP405101PZN
CSB-BP405101PZN
CSB-MP405101PZN
CSB-EP405101PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP405102PZN
CSB-EP405102PZN
CSB-BP405102PZN
CSB-MP405102PZN
CSB-EP405102PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP405103PZN
CSB-EP405103PZN
CSB-BP405103PZN
CSB-MP405103PZN
CSB-EP405103PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA pseudouridine synthase B (truB) CSB-YP405104PZN
CSB-EP405104PZN
CSB-BP405104PZN
CSB-MP405104PZN
CSB-EP405104PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP405105PZN
CSB-EP405105PZN
CSB-BP405105PZN
CSB-MP405105PZN
CSB-EP405105PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida SsrA-binding protein (smpB) CSB-YP405106PZN
CSB-EP405106PZN
CSB-BP405106PZN
CSB-MP405106PZN
CSB-EP405106PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Leucine--tRNA ligase (leuS), partial CSB-YP405107PZN
CSB-EP405107PZN
CSB-BP405107PZN
CSB-MP405107PZN
CSB-EP405107PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP405108PZN
CSB-EP405108PZN
CSB-BP405108PZN
CSB-MP405108PZN
CSB-EP405108PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP405109PZN
CSB-EP405109PZN
CSB-BP405109PZN
CSB-MP405109PZN
CSB-EP405109PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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