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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosipho melanesiensis UPF0296 protein Tmel_0100 (Tmel_0100) CSB-YP406692TKD
CSB-EP406692TKD
CSB-BP406692TKD
CSB-MP406692TKD
CSB-EP406692TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP406693TKD
CSB-EP406693TKD
CSB-BP406693TKD
CSB-MP406693TKD
CSB-EP406693TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis SsrA-binding protein (smpB) CSB-YP406694TKD
CSB-EP406694TKD
CSB-BP406694TKD
CSB-MP406694TKD
CSB-EP406694TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Orotate phosphoribosyltransferase (pyrE) CSB-YP406695TKD
CSB-EP406695TKD
CSB-BP406695TKD
CSB-MP406695TKD
CSB-EP406695TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis UPF0173 metal-dependent hydrolase Tmel_0315 (Tmel_0315) CSB-YP406696TKD
CSB-EP406696TKD
CSB-BP406696TKD
CSB-MP406696TKD
CSB-EP406696TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP406697TKD
CSB-EP406697TKD
CSB-BP406697TKD
CSB-MP406697TKD
CSB-EP406697TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP406698TKD
CSB-EP406698TKD
CSB-BP406698TKD
CSB-MP406698TKD
CSB-EP406698TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Protein translocase subunit SecA (secA), partial CSB-YP406699TKD
CSB-EP406699TKD
CSB-BP406699TKD
CSB-MP406699TKD
CSB-EP406699TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Elongation factor P (efp) CSB-YP406700TKD
CSB-EP406700TKD
CSB-BP406700TKD
CSB-MP406700TKD
CSB-EP406700TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 30S ribosomal protein S10 (rpsJ) CSB-YP406701TKD
CSB-EP406701TKD
CSB-BP406701TKD
CSB-MP406701TKD
CSB-EP406701TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Adenylate kinase (adk) CSB-YP406702TKD
CSB-EP406702TKD
CSB-BP406702TKD
CSB-MP406702TKD
CSB-EP406702TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP406703TKD
CSB-EP406703TKD
CSB-BP406703TKD
CSB-MP406703TKD
CSB-EP406703TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP406704TKD
CSB-EP406704TKD
CSB-BP406704TKD
CSB-MP406704TKD
CSB-EP406704TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Glutamate--tRNA ligase 2 (gltX2) CSB-YP406705TKD
CSB-EP406705TKD
CSB-BP406705TKD
CSB-MP406705TKD
CSB-EP406705TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Type III pantothenate kinase (coaX) CSB-YP406706TKD
CSB-EP406706TKD
CSB-BP406706TKD
CSB-MP406706TKD
CSB-EP406706TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis DNA ligase (ligA), partial CSB-YP406707TKD
CSB-EP406707TKD
CSB-BP406707TKD
CSB-MP406707TKD
CSB-EP406707TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Pantothenate synthetase (panC) CSB-YP406708TKD
CSB-EP406708TKD
CSB-BP406708TKD
CSB-MP406708TKD
CSB-EP406708TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis GTPase Der (der) CSB-YP406709TKD
CSB-EP406709TKD
CSB-BP406709TKD
CSB-MP406709TKD
CSB-EP406709TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 30S ribosomal protein S16 (rpsP) CSB-YP406710TKD
CSB-EP406710TKD
CSB-BP406710TKD
CSB-MP406710TKD
CSB-EP406710TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Dihydrodipicolinate reductase (dapB) CSB-YP406711TKD
CSB-EP406711TKD
CSB-BP406711TKD
CSB-MP406711TKD
CSB-EP406711TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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