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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis 30S ribosomal protein S9P (rps9p) CSB-YP407892MNR
CSB-EP407892MNR
CSB-BP407892MNR
CSB-MP407892MNR
CSB-EP407892MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose 1,5-bisphosphate isomerase (MmarC7_0597) CSB-YP407893MNR
CSB-EP407893MNR
CSB-BP407893MNR
CSB-MP407893MNR
CSB-EP407893MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP407894MNR
CSB-EP407894MNR
CSB-BP407894MNR
CSB-MP407894MNR
CSB-EP407894MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L14P (rpl14p) CSB-YP407895MNR
CSB-EP407895MNR
CSB-BP407895MNR
CSB-MP407895MNR
CSB-EP407895MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis tRNA (cytidine (56)-2'-O)-methyltransferase CSB-YP407896MNR
CSB-EP407896MNR
CSB-BP407896MNR
CSB-MP407896MNR
CSB-EP407896MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phenylalanine--tRNA ligase alpha subunit (pheS), partial CSB-YP407897MNR
CSB-EP407897MNR
CSB-BP407897MNR
CSB-MP407897MNR
CSB-EP407897MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L23P (rpl23p) CSB-YP407898MNR
CSB-EP407898MNR
CSB-BP407898MNR
CSB-MP407898MNR
CSB-EP407898MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Signal recognition 54 kDa protein (srp54) CSB-YP407899MNR
CSB-EP407899MNR
CSB-BP407899MNR
CSB-MP407899MNR
CSB-EP407899MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nicotinamide-nucleotide adenylyltransferase (MmarC7_0825) CSB-YP407900MNR
CSB-EP407900MNR
CSB-BP407900MNR
CSB-MP407900MNR
CSB-EP407900MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartate carbamoyltransferase (pyrB) CSB-YP407901MNR
CSB-EP407901MNR
CSB-BP407901MNR
CSB-MP407901MNR
CSB-EP407901MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA polymerase II large subunit (polC), partial CSB-YP407902MNR
CSB-EP407902MNR
CSB-BP407902MNR
CSB-MP407902MNR
CSB-EP407902MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 6 (eif6) CSB-YP407903MNR
CSB-EP407903MNR
CSB-BP407903MNR
CSB-MP407903MNR
CSB-EP407903MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP407904MNR
CSB-EP407904MNR
CSB-BP407904MNR
CSB-MP407904MNR
CSB-EP407904MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L40e (rpl40e) CSB-YP407905MNR
CSB-EP407905MNR
CSB-BP407905MNR
CSB-MP407905MNR
CSB-EP407905MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP407906MNR
CSB-EP407906MNR
CSB-BP407906MNR
CSB-MP407906MNR
CSB-EP407906MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Homoserine kinase (thrB) CSB-YP407907MNR
CSB-EP407907MNR
CSB-BP407907MNR
CSB-MP407907MNR
CSB-EP407907MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Replication factor C large subunit (rfcL) CSB-YP407908MNR
CSB-EP407908MNR
CSB-BP407908MNR
CSB-MP407908MNR
CSB-EP407908MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Enolase (eno) CSB-YP407909MNR
CSB-EP407909MNR
CSB-BP407909MNR
CSB-MP407909MNR
CSB-EP407909MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Replication factor C small subunit (rfcS) CSB-YP407910MNR
CSB-EP407910MNR
CSB-BP407910MNR
CSB-MP407910MNR
CSB-EP407910MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP407911MNR
CSB-EP407911MNR
CSB-BP407911MNR
CSB-MP407911MNR
CSB-EP407911MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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