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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Psychrobacter sp. Methionine--tRNA ligase (metG), partial CSB-YP406872PZR
CSB-EP406872PZR
CSB-BP406872PZR
CSB-MP406872PZR
CSB-EP406872PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Bifunctional protein FolD (folD) CSB-YP406873PZR
CSB-EP406873PZR
CSB-BP406873PZR
CSB-MP406873PZR
CSB-EP406873PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Histidine--tRNA ligase (hisS) CSB-YP406874PZR
CSB-EP406874PZR
CSB-BP406874PZR
CSB-MP406874PZR
CSB-EP406874PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Protein translocase subunit SecA (secA), partial CSB-YP406875PZR
CSB-EP406875PZR
CSB-BP406875PZR
CSB-MP406875PZR
CSB-EP406875PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. D-tyrosyl-tRNA (Tyr) deacylase CSB-YP406876PZR
CSB-EP406876PZR
CSB-BP406876PZR
CSB-MP406876PZR
CSB-EP406876PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Dihydrodipicolinate synthase (dapA) CSB-YP406877PZR
CSB-EP406877PZR
CSB-BP406877PZR
CSB-MP406877PZR
CSB-EP406877PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP406878PZR
CSB-EP406878PZR
CSB-BP406878PZR
CSB-MP406878PZR
CSB-EP406878PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP406879PZR
CSB-EP406879PZR
CSB-BP406879PZR
CSB-MP406879PZR
CSB-EP406879PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. tRNA modification GTPase MnmE (mnmE) CSB-YP406880PZR
CSB-EP406880PZR
CSB-BP406880PZR
CSB-MP406880PZR
CSB-EP406880PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP406881MOO
CSB-EP406881MOO
CSB-BP406881MOO
CSB-MP406881MOO
CSB-EP406881MOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP406882MOO
CSB-EP406882MOO
CSB-BP406882MOO
CSB-MP406882MOO
CSB-EP406882MOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sistrurus catenatus edwardsii Fused toxin protein CSB-YP406883SZP
CSB-EP406883SZP
CSB-BP406883SZP
CSB-MP406883SZP
CSB-EP406883SZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inosose dehydratase (iolE) CSB-YP406884LMH
CSB-EP406884LMH
CSB-BP406884LMH
CSB-MP406884LMH
CSB-EP406884LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP406885GEO1
CSB-EP406885GEO1
CSB-BP406885GEO1
CSB-MP406885GEO1
CSB-EP406885GEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP406886GEO
CSB-EP406886GEO
CSB-BP406886GEO
CSB-MP406886GEO
CSB-EP406886GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Photosystem Q (B) protein, partial CSB-YP406887GEO1
CSB-EP406887GEO1
CSB-BP406887GEO1
CSB-MP406887GEO1
CSB-EP406887GEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP406888GEO
CSB-EP406888GEO
CSB-BP406888GEO
CSB-MP406888GEO
CSB-EP406888GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP406889GEO
CSB-EP406889GEO
CSB-BP406889GEO
CSB-MP406889GEO
CSB-EP406889GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP406890GEO1
CSB-EP406890GEO1
CSB-BP406890GEO1
CSB-MP406890GEO1
CSB-EP406890GEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum UPF0102 protein FP0059 (FP0059) CSB-YP406891FDU
CSB-EP406891FDU
CSB-BP406891FDU
CSB-MP406891FDU
CSB-EP406891FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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