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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Alkaliphilus metalliredigens N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP407672AUP
CSB-EP407672AUP
CSB-BP407672AUP
CSB-MP407672AUP
CSB-EP407672AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Asparagine--tRNA ligase (asnS) CSB-YP407673AUP
CSB-EP407673AUP
CSB-BP407673AUP
CSB-MP407673AUP
CSB-EP407673AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Octanoyltransferase (lipB) CSB-YP407674AUP
CSB-EP407674AUP
CSB-BP407674AUP
CSB-MP407674AUP
CSB-EP407674AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L13 (rplM) CSB-YP407675AUP
CSB-EP407675AUP
CSB-BP407675AUP
CSB-MP407675AUP
CSB-EP407675AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L36 (rpmJ) CSB-YP407676AUP
CSB-EP407676AUP
CSB-BP407676AUP
CSB-MP407676AUP
CSB-EP407676AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 30S ribosomal protein S5 (rpsE) CSB-YP407677AUP
CSB-EP407677AUP
CSB-BP407677AUP
CSB-MP407677AUP
CSB-EP407677AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 30S ribosomal protein S14 type Z (rpsZ) CSB-YP407678AUP
CSB-EP407678AUP
CSB-BP407678AUP
CSB-MP407678AUP
CSB-EP407678AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 30S ribosomal protein S17 (rpsQ) CSB-YP407679AUP
CSB-EP407679AUP
CSB-BP407679AUP
CSB-MP407679AUP
CSB-EP407679AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 30S ribosomal protein S12 (rpsL) CSB-YP407680AUP
CSB-EP407680AUP
CSB-BP407680AUP
CSB-MP407680AUP
CSB-EP407680AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP407681AUP
CSB-EP407681AUP
CSB-BP407681AUP
CSB-MP407681AUP
CSB-EP407681AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens UPF0173 metal-dependent hydrolase Amet_4625 (Amet_4625) CSB-YP407682AUP
CSB-EP407682AUP
CSB-BP407682AUP
CSB-MP407682AUP
CSB-EP407682AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine--tRNA ligase (serS) CSB-YP407683SUI
CSB-EP407683SUI
CSB-BP407683SUI
CSB-MP407683SUI
CSB-EP407683SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-ribose pyranase (rbsD) CSB-YP407684SUI
CSB-EP407684SUI
CSB-BP407684SUI
CSB-MP407684SUI
CSB-EP407684SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Initiation-control protein yabA (SaurJH1_0520) CSB-YP407685SUI
CSB-EP407685SUI
CSB-BP407685SUI
CSB-MP407685SUI
CSB-EP407685SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP407686SUI
CSB-EP407686SUI
CSB-BP407686SUI
CSB-MP407686SUI
CSB-EP407686SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 1 (rpmG1) CSB-YP407687SUI
CSB-EP407687SUI
CSB-BP407687SUI
CSB-MP407687SUI
CSB-EP407687SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molecular chaperone Hsp31 and glyoxalase 3 (hchA) CSB-YP407688SUI
CSB-EP407688SUI
CSB-BP407688SUI
CSB-MP407688SUI
CSB-EP407688SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhE2 (mnhE2), partial CSB-YP407689SUI1
CSB-EP407689SUI1
CSB-BP407689SUI1
CSB-MP407689SUI1
CSB-EP407689SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0178 protein SaurJH1_0721 (SaurJH1_0721) CSB-YP407690SUI
CSB-EP407690SUI
CSB-BP407690SUI
CSB-MP407690SUI
CSB-EP407690SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidinol-phosphate aminotransferase (hisC) CSB-YP407691SUI
CSB-EP407691SUI
CSB-BP407691SUI
CSB-MP407691SUI
CSB-EP407691SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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