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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus aeolicus Translation initiation factor 2 subunit gamma (eIF2G) CSB-YP408672MNO
CSB-EP408672MNO
CSB-BP408672MNO
CSB-MP408672MNO
CSB-EP408672MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 30S ribosomal protein S24e (rps24e) CSB-YP408673MNO
CSB-EP408673MNO
CSB-BP408673MNO
CSB-MP408673MNO
CSB-EP408673MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP408674MNO
CSB-EP408674MNO
CSB-BP408674MNO
CSB-MP408674MNO
CSB-EP408674MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP408675MNO
CSB-EP408675MNO
CSB-BP408675MNO
CSB-MP408675MNO
CSB-EP408675MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Ribonuclease HII (rnhB) CSB-YP408676MNO
CSB-EP408676MNO
CSB-BP408676MNO
CSB-MP408676MNO
CSB-EP408676MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP408677MNO
CSB-EP408677MNO
CSB-BP408677MNO
CSB-MP408677MNO
CSB-EP408677MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Chorismate synthase (aroC) CSB-YP408678MNO
CSB-EP408678MNO
CSB-BP408678MNO
CSB-MP408678MNO
CSB-EP408678MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Uridylate kinase (pyrH) CSB-YP408679MNO
CSB-EP408679MNO
CSB-BP408679MNO
CSB-MP408679MNO
CSB-EP408679MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 7-cyano-7-deazaguanine synthase (queC) CSB-YP408680MNO
CSB-EP408680MNO
CSB-BP408680MNO
CSB-MP408680MNO
CSB-EP408680MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP408681MNO
CSB-EP408681MNO
CSB-BP408681MNO
CSB-MP408681MNO
CSB-EP408681MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Homoserine kinase (thrB) CSB-YP408682MNO
CSB-EP408682MNO
CSB-BP408682MNO
CSB-MP408682MNO
CSB-EP408682MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Dihydrodipicolinate synthase (dapA) CSB-YP408683MNO
CSB-EP408683MNO
CSB-BP408683MNO
CSB-MP408683MNO
CSB-EP408683MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus DNA-binding protein Maeo_0998 (Maeo_0998) CSB-YP408684MNO
CSB-EP408684MNO
CSB-BP408684MNO
CSB-MP408684MNO
CSB-EP408684MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus O-phosphoserine--tRNA (Cys) ligase, partial CSB-YP408685MNO
CSB-EP408685MNO
CSB-BP408685MNO
CSB-MP408685MNO
CSB-EP408685MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Anthranilate phosphoribosyltransferase (trpD) CSB-YP408686MNO
CSB-EP408686MNO
CSB-BP408686MNO
CSB-MP408686MNO
CSB-EP408686MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable tRNA pseudouridine synthase B (truB) CSB-YP408687MNO
CSB-EP408687MNO
CSB-BP408687MNO
CSB-MP408687MNO
CSB-EP408687MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus F420-dependent methylenetetrahydromethanopterin dehydrogenase (mtd) CSB-YP408688MNO
CSB-EP408688MNO
CSB-BP408688MNO
CSB-MP408688MNO
CSB-EP408688MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L29P (rpl29p) CSB-YP408689MNO
CSB-EP408689MNO
CSB-BP408689MNO
CSB-MP408689MNO
CSB-EP408689MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L6P (rpl6p) CSB-YP408690MNO
CSB-EP408690MNO
CSB-BP408690MNO
CSB-MP408690MNO
CSB-EP408690MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable deoxycytidine triphosphate deaminase (dcd) CSB-YP408691MNO
CSB-EP408691MNO
CSB-BP408691MNO
CSB-MP408691MNO
CSB-EP408691MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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