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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis UPF0285 protein MmarC7_1666 (MmarC7_1666) CSB-YP407912MNR
CSB-EP407912MNR
CSB-BP407912MNR
CSB-MP407912MNR
CSB-EP407912MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative HTH-type transcriptional regulatory protein MmarC7_1702 (MmarC7_1702) CSB-YP407913MNR
CSB-EP407913MNR
CSB-BP407913MNR
CSB-MP407913MNR
CSB-EP407913MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0758 protein Asuc_0013 (Asuc_0013) CSB-YP407914AUG
CSB-EP407914AUG
CSB-BP407914AUG
CSB-MP407914AUG
CSB-EP407914AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L10 (rplJ) CSB-YP407915AUG
CSB-EP407915AUG
CSB-BP407915AUG
CSB-MP407915AUG
CSB-EP407915AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Proline--tRNA ligase (proS), partial CSB-YP407916AUG
CSB-EP407916AUG
CSB-BP407916AUG
CSB-MP407916AUG
CSB-EP407916AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Large-conductance mechanosensitive channel (mscL), partial CSB-YP407917AUG1
CSB-EP407917AUG1
CSB-BP407917AUG1
CSB-MP407917AUG1
CSB-EP407917AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes ATP synthase epsilon chain (atpC) CSB-YP407918AUG
CSB-EP407918AUG
CSB-BP407918AUG
CSB-MP407918AUG
CSB-EP407918AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP407919AUG
CSB-EP407919AUG
CSB-BP407919AUG
CSB-MP407919AUG
CSB-EP407919AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Protein CyaY (cyaY) CSB-YP407920AUG
CSB-EP407920AUG
CSB-BP407920AUG
CSB-MP407920AUG
CSB-EP407920AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Sulfurtransferase TusA homolog (tusA) CSB-YP407921AUG
CSB-EP407921AUG
CSB-BP407921AUG
CSB-MP407921AUG
CSB-EP407921AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phosphoglycerate kinase (pgk) CSB-YP407922AUG
CSB-EP407922AUG
CSB-BP407922AUG
CSB-MP407922AUG
CSB-EP407922AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Glutamate--tRNA ligase (gltX) CSB-YP407923AUG
CSB-EP407923AUG
CSB-BP407923AUG
CSB-MP407923AUG
CSB-EP407923AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Na (+)-translocating NADH-quinone reductase subunit A CSB-YP407924AUG
CSB-EP407924AUG
CSB-BP407924AUG
CSB-MP407924AUG
CSB-EP407924AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal protein L11 methyltransferase (prmA) CSB-YP407925AUG
CSB-EP407925AUG
CSB-BP407925AUG
CSB-MP407925AUG
CSB-EP407925AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phosphopantetheine adenylyltransferase (coaD) CSB-YP407926AUG
CSB-EP407926AUG
CSB-BP407926AUG
CSB-MP407926AUG
CSB-EP407926AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S18 (rpsR) CSB-YP407927AUG
CSB-EP407927AUG
CSB-BP407927AUG
CSB-MP407927AUG
CSB-EP407927AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S2 (rpsB) CSB-YP407928AUG
CSB-EP407928AUG
CSB-BP407928AUG
CSB-MP407928AUG
CSB-EP407928AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribonuclease H (rnhA) CSB-YP407929AUG
CSB-EP407929AUG
CSB-BP407929AUG
CSB-MP407929AUG
CSB-EP407929AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0042 nucleotide-binding protein Asuc_0930 (Asuc_0930) CSB-YP407930AUG
CSB-EP407930AUG
CSB-BP407930AUG
CSB-MP407930AUG
CSB-EP407930AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP407931AUG
CSB-EP407931AUG
CSB-BP407931AUG
CSB-MP407931AUG
CSB-EP407931AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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