Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nitratiruptor sp. RNA pyrophosphohydrolase (rppH) CSB-YP409012NFA
CSB-EP409012NFA
CSB-BP409012NFA
CSB-MP409012NFA
CSB-EP409012NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Chaperone protein DnaJ (dnaJ) CSB-YP409013NFA
CSB-EP409013NFA
CSB-BP409013NFA
CSB-MP409013NFA
CSB-EP409013NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 5'-nucleotidase surE (surE) CSB-YP409014NFA
CSB-EP409014NFA
CSB-BP409014NFA
CSB-MP409014NFA
CSB-EP409014NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 2-isopropylmalate synthase (leuA), partial CSB-YP409015NFA
CSB-EP409015NFA
CSB-BP409015NFA
CSB-MP409015NFA
CSB-EP409015NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. tRNA pseudouridine synthase B (truB) CSB-YP409016NFA
CSB-EP409016NFA
CSB-BP409016NFA
CSB-MP409016NFA
CSB-EP409016NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. tRNA (guanine-N (1)-)-methyltransferase CSB-YP409017NFA
CSB-EP409017NFA
CSB-BP409017NFA
CSB-MP409017NFA
CSB-EP409017NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Pantothenate synthetase (panC) CSB-YP409018NFA
CSB-EP409018NFA
CSB-BP409018NFA
CSB-MP409018NFA
CSB-EP409018NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP409019NFA
CSB-EP409019NFA
CSB-BP409019NFA
CSB-MP409019NFA
CSB-EP409019NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Biotin synthase (bioB) CSB-YP409020NFA
CSB-EP409020NFA
CSB-BP409020NFA
CSB-MP409020NFA
CSB-EP409020NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP409021SUZ
CSB-EP409021SUZ
CSB-BP409021SUZ
CSB-MP409021SUZ
CSB-EP409021SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Lipoprotein signal peptidase (lspA), partial CSB-YP409022SUZ1
CSB-EP409022SUZ1
CSB-BP409022SUZ1
CSB-MP409022SUZ1
CSB-EP409022SUZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Na (+)/H (+) antiporter nhaA, partial CSB-YP409023SUZ
CSB-EP409023SUZ
CSB-BP409023SUZ
CSB-MP409023SUZ
CSB-EP409023SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. 30S ribosomal protein S7 (rpsG) CSB-YP409024SUZ
CSB-EP409024SUZ
CSB-BP409024SUZ
CSB-MP409024SUZ
CSB-EP409024SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Adenylosuccinate synthetase (purA) CSB-YP409025SUZ
CSB-EP409025SUZ
CSB-BP409025SUZ
CSB-MP409025SUZ
CSB-EP409025SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. UPF0234 protein SUN_0226 (SUN_0226) CSB-YP409026SUZ
CSB-EP409026SUZ
CSB-BP409026SUZ
CSB-MP409026SUZ
CSB-EP409026SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Pantothenate synthetase (panC) CSB-YP409027SUZ
CSB-EP409027SUZ
CSB-BP409027SUZ
CSB-MP409027SUZ
CSB-EP409027SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP409028SUZ
CSB-EP409028SUZ
CSB-BP409028SUZ
CSB-MP409028SUZ
CSB-EP409028SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. DNA ligase (ligA), partial CSB-YP409029SUZ
CSB-EP409029SUZ
CSB-BP409029SUZ
CSB-MP409029SUZ
CSB-EP409029SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP409030SUZ
CSB-EP409030SUZ
CSB-BP409030SUZ
CSB-MP409030SUZ
CSB-EP409030SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Proline--tRNA ligase (proS), partial CSB-YP409031SUZ
CSB-EP409031SUZ
CSB-BP409031SUZ
CSB-MP409031SUZ
CSB-EP409031SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>