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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus succinogenes Periplasmic nitrate reductase (napA), partial CSB-YP410413AUG
CSB-EP410413AUG
CSB-BP410413AUG
CSB-MP410413AUG
CSB-EP410413AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Elongation factor P (efp) CSB-YP410414AUG
CSB-EP410414AUG
CSB-BP410414AUG
CSB-MP410414AUG
CSB-EP410414AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP410415MNJ1
CSB-EP410415MNJ1
CSB-BP410415MNJ1
CSB-MP410415MNJ1
CSB-EP410415MNJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP410416MNJ
CSB-EP410416MNJ
CSB-BP410416MNJ
CSB-MP410416MNJ
CSB-EP410416MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Urease subunit beta (ureB) CSB-YP410417MNJ
CSB-EP410417MNJ
CSB-BP410417MNJ
CSB-MP410417MNJ
CSB-EP410417MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Ribosome maturation factor RimP (rimP) CSB-YP410418MNJ
CSB-EP410418MNJ
CSB-BP410418MNJ
CSB-MP410418MNJ
CSB-EP410418MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP410419MNJ
CSB-EP410419MNJ
CSB-BP410419MNJ
CSB-MP410419MNJ
CSB-EP410419MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP410420MNJ
CSB-EP410420MNJ
CSB-BP410420MNJ
CSB-MP410420MNJ
CSB-EP410420MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP410421MNJ
CSB-EP410421MNJ
CSB-BP410421MNJ
CSB-MP410421MNJ
CSB-EP410421MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Adenine deaminase (Mmwyl1_1922) CSB-YP410422MNJ
CSB-EP410422MNJ
CSB-BP410422MNJ
CSB-MP410422MNJ
CSB-EP410422MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. ATP synthase subunit alpha 1 (atpA1), partial CSB-YP410423MNJ
CSB-EP410423MNJ
CSB-BP410423MNJ
CSB-MP410423MNJ
CSB-EP410423MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. LexA repressor (lexA) CSB-YP410424MNJ
CSB-EP410424MNJ
CSB-BP410424MNJ
CSB-MP410424MNJ
CSB-EP410424MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Aspartate--tRNA ligase (aspS), partial CSB-YP410425MNJ
CSB-EP410425MNJ
CSB-BP410425MNJ
CSB-MP410425MNJ
CSB-EP410425MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. UPF0178 protein Mmwyl1_2258 (Mmwyl1_2258) CSB-YP410426MNJ
CSB-EP410426MNJ
CSB-BP410426MNJ
CSB-MP410426MNJ
CSB-EP410426MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Chaperone protein htpG (htpG), partial CSB-YP410427MNJ
CSB-EP410427MNJ
CSB-BP410427MNJ
CSB-MP410427MNJ
CSB-EP410427MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Threonine--tRNA ligase (thrS), partial CSB-YP410428MNJ
CSB-EP410428MNJ
CSB-BP410428MNJ
CSB-MP410428MNJ
CSB-EP410428MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Adenylosuccinate synthetase (purA) CSB-YP410429MNJ
CSB-EP410429MNJ
CSB-BP410429MNJ
CSB-MP410429MNJ
CSB-EP410429MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP410430MNJ
CSB-EP410430MNJ
CSB-BP410430MNJ
CSB-MP410430MNJ
CSB-EP410430MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Glutamate--tRNA ligase (gltX) CSB-YP410431MNJ
CSB-EP410431MNJ
CSB-BP410431MNJ
CSB-MP410431MNJ
CSB-EP410431MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP410432MNJ
CSB-EP410432MNJ
CSB-BP410432MNJ
CSB-MP410432MNJ
CSB-EP410432MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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