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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus aeolicus Elongation factor 1-beta (ef1b) CSB-YP409412MNO
CSB-EP409412MNO
CSB-BP409412MNO
CSB-MP409412MNO
CSB-EP409412MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 30S ribosomal protein S5P (rps5p) CSB-YP409413MNO
CSB-EP409413MNO
CSB-BP409413MNO
CSB-MP409413MNO
CSB-EP409413MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP409414MNO
CSB-EP409414MNO
CSB-BP409414MNO
CSB-MP409414MNO
CSB-EP409414MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L15e (rpl15e) CSB-YP409415MNO
CSB-EP409415MNO
CSB-BP409415MNO
CSB-MP409415MNO
CSB-EP409415MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Tryptophan synthase alpha chain (trpA) CSB-YP409416PZL
CSB-EP409416PZL
CSB-BP409416PZL
CSB-MP409416PZL
CSB-EP409416PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Agmatine deiminase (aguA) CSB-YP409417PZL
CSB-EP409417PZL
CSB-BP409417PZL
CSB-MP409417PZL
CSB-EP409417PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP409418PZL
CSB-EP409418PZL
CSB-BP409418PZL
CSB-MP409418PZL
CSB-EP409418PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0301 protein PSPA7_0505 (PSPA7_0505) CSB-YP409419PZL
CSB-EP409419PZL
CSB-BP409419PZL
CSB-MP409419PZL
CSB-EP409419PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP409420PZL1
CSB-EP409420PZL1
CSB-BP409420PZL1
CSB-MP409420PZL1
CSB-EP409420PZL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Indole-3-glycerol phosphate synthase (trpC) CSB-YP409421PZL
CSB-EP409421PZL
CSB-BP409421PZL
CSB-MP409421PZL
CSB-EP409421PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S10 (rpsJ) CSB-YP409422PZL
CSB-EP409422PZL
CSB-BP409422PZL
CSB-MP409422PZL
CSB-EP409422PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L22 (rplV) CSB-YP409423PZL
CSB-EP409423PZL
CSB-BP409423PZL
CSB-MP409423PZL
CSB-EP409423PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S14 (rpsN) CSB-YP409424PZL
CSB-EP409424PZL
CSB-BP409424PZL
CSB-MP409424PZL
CSB-EP409424PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S13 (rpsM) CSB-YP409425PZL
CSB-EP409425PZL
CSB-BP409425PZL
CSB-MP409425PZL
CSB-EP409425PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP409426PZL
CSB-EP409426PZL
CSB-BP409426PZL
CSB-MP409426PZL
CSB-EP409426PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0250 protein PSPA7_1111 (PSPA7_1111) CSB-YP409427PZL
CSB-EP409427PZL
CSB-BP409427PZL
CSB-MP409427PZL
CSB-EP409427PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable cytosol aminopeptidase (pepA) CSB-YP409428PZL
CSB-EP409428PZL
CSB-BP409428PZL
CSB-MP409428PZL
CSB-EP409428PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Histidine--tRNA ligase (hisS) CSB-YP409429PZL
CSB-EP409429PZL
CSB-BP409429PZL
CSB-MP409429PZL
CSB-EP409429PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Lysine--tRNA ligase (lysS), partial CSB-YP409430PZL
CSB-EP409430PZL
CSB-BP409430PZL
CSB-MP409430PZL
CSB-EP409430PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Uridylate kinase (pyrH) CSB-YP409431PZL
CSB-EP409431PZL
CSB-BP409431PZL
CSB-MP409431PZL
CSB-EP409431PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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