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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae subsp. pneumoniae Macrodomain Ter protein (matP) CSB-YP409172KAX
CSB-EP409172KAX
CSB-BP409172KAX
CSB-MP409172KAX
CSB-EP409172KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable malonic semialdehyde reductase RutE (rutE) CSB-YP409173KAX
CSB-EP409173KAX
CSB-BP409173KAX
CSB-MP409173KAX
CSB-EP409173KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0227 protein KPN78578_10770 (KPN78578_10770) CSB-YP409174KAX
CSB-EP409174KAX
CSB-BP409174KAX
CSB-MP409174KAX
CSB-EP409174KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Probable intracellular septation protein A (KPN78578_12170), partial CSB-YP409175KAX1
CSB-EP409175KAX1
CSB-BP409175KAX1
CSB-MP409175KAX1
CSB-EP409175KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Phosphoribosyl-dephospho-CoA transferase (mdcG) CSB-YP409176KAX
CSB-EP409176KAX
CSB-BP409176KAX
CSB-MP409176KAX
CSB-EP409176KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Pyrroloquinoline-quinone synthase (pqqC) CSB-YP409177KAX
CSB-EP409177KAX
CSB-BP409177KAX
CSB-MP409177KAX
CSB-EP409177KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP409178KAX
CSB-EP409178KAX
CSB-BP409178KAX
CSB-MP409178KAX
CSB-EP409178KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, partial CSB-YP409179KAX
CSB-EP409179KAX
CSB-BP409179KAX
CSB-MP409179KAX
CSB-EP409179KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP409180KAX
CSB-EP409180KAX
CSB-BP409180KAX
CSB-MP409180KAX
CSB-EP409180KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L20 (rplT) CSB-YP409181KAX
CSB-EP409181KAX
CSB-BP409181KAX
CSB-MP409181KAX
CSB-EP409181KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP409182KAX
CSB-EP409182KAX
CSB-BP409182KAX
CSB-MP409182KAX
CSB-EP409182KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae UPF0745 protein KPN78578_22820 (KPN78578_22820) CSB-YP409183KAX
CSB-EP409183KAX
CSB-BP409183KAX
CSB-MP409183KAX
CSB-EP409183KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae ProP effector (proQ) CSB-YP409184KAX
CSB-EP409184KAX
CSB-BP409184KAX
CSB-MP409184KAX
CSB-EP409184KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Copper homeostasis protein CutC (cutC) CSB-YP409185KAX
CSB-EP409185KAX
CSB-BP409185KAX
CSB-MP409185KAX
CSB-EP409185KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Cobalamin synthase (cobS), partial CSB-YP409186KAX1
CSB-EP409186KAX1
CSB-BP409186KAX1
CSB-MP409186KAX1
CSB-EP409186KAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Deoxycytidine triphosphate deaminase (dcd) CSB-YP409187KAX
CSB-EP409187KAX
CSB-BP409187KAX
CSB-MP409187KAX
CSB-EP409187KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 50S ribosomal protein L25 (rplY) CSB-YP409188KAX
CSB-EP409188KAX
CSB-BP409188KAX
CSB-MP409188KAX
CSB-EP409188KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP409189KAX
CSB-EP409189KAX
CSB-BP409189KAX
CSB-MP409189KAX
CSB-EP409189KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Chorismate synthase (aroC) CSB-YP409190KAX
CSB-EP409190KAX
CSB-BP409190KAX
CSB-MP409190KAX
CSB-EP409190KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Glutamate--tRNA ligase (gltX) CSB-YP409191KAX
CSB-EP409191KAX
CSB-BP409191KAX
CSB-MP409191KAX
CSB-EP409191KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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