Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Alkaliphilus metalliredigens Acetate kinase (ackA) CSB-YP409252AUP
CSB-EP409252AUP
CSB-BP409252AUP
CSB-MP409252AUP
CSB-EP409252AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP409253AUP
CSB-EP409253AUP
CSB-BP409253AUP
CSB-MP409253AUP
CSB-EP409253AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens GTPase Der (der) CSB-YP409254AUP
CSB-EP409254AUP
CSB-BP409254AUP
CSB-MP409254AUP
CSB-EP409254AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP409255AUP
CSB-EP409255AUP
CSB-BP409255AUP
CSB-MP409255AUP
CSB-EP409255AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens UPF0271 protein Amet_2977 (Amet_2977) CSB-YP409256AUP
CSB-EP409256AUP
CSB-BP409256AUP
CSB-MP409256AUP
CSB-EP409256AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Elongation factor 4 (lepA), partial CSB-YP409257AUP
CSB-EP409257AUP
CSB-BP409257AUP
CSB-MP409257AUP
CSB-EP409257AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Biotin synthase (bioB) CSB-YP409258AUP
CSB-EP409258AUP
CSB-BP409258AUP
CSB-MP409258AUP
CSB-EP409258AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Acetylglutamate kinase (argB) CSB-YP409259AUP
CSB-EP409259AUP
CSB-BP409259AUP
CSB-MP409259AUP
CSB-EP409259AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Nitrogenase iron protein (nifH) CSB-YP409260AUP
CSB-EP409260AUP
CSB-BP409260AUP
CSB-MP409260AUP
CSB-EP409260AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP409261AUP
CSB-EP409261AUP
CSB-BP409261AUP
CSB-MP409261AUP
CSB-EP409261AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens UvrABC system protein C (uvrC), partial CSB-YP409262AUP
CSB-EP409262AUP
CSB-BP409262AUP
CSB-MP409262AUP
CSB-EP409262AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Phosphomethylpyrimidine synthase (thiC) CSB-YP409263AUP
CSB-EP409263AUP
CSB-BP409263AUP
CSB-MP409263AUP
CSB-EP409263AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP409264AUP
CSB-EP409264AUP
CSB-BP409264AUP
CSB-MP409264AUP
CSB-EP409264AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L6 (rplF) CSB-YP409265AUP
CSB-EP409265AUP
CSB-BP409265AUP
CSB-MP409265AUP
CSB-EP409265AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens 50S ribosomal protein L22 (rplV) CSB-YP409266AUP
CSB-EP409266AUP
CSB-BP409266AUP
CSB-MP409266AUP
CSB-EP409266AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP409267AUP
CSB-EP409267AUP
CSB-BP409267AUP
CSB-MP409267AUP
CSB-EP409267AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens ATP-dependent zinc metalloprotease FtsH 2 (ftsH2), partial CSB-YP409268AUP
CSB-EP409268AUP
CSB-BP409268AUP
CSB-MP409268AUP
CSB-EP409268AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Putative membrane protein insertion efficiency factor (Amet_4800) CSB-YP409269AUP
CSB-EP409269AUP
CSB-BP409269AUP
CSB-MP409269AUP
CSB-EP409269AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial CSB-YP409270SUI1
CSB-EP409270SUI1
CSB-BP409270SUI1
CSB-MP409270SUI1
CSB-EP409270SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative septation protein spoVG (spoVG) CSB-YP409271SUI
CSB-EP409271SUI
CSB-BP409271SUI
CSB-MP409271SUI
CSB-EP409271SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>