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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Botryotinia fuckeliana ATP-dependent DNA helicase mph1 (mph1), partial CSB-YP409112BVD
CSB-EP409112BVD
CSB-BP409112BVD
CSB-MP409112BVD
CSB-EP409112BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana 40S ribosomal protein S0 (rps0) CSB-YP409113BVD
CSB-EP409113BVD
CSB-BP409113BVD
CSB-MP409113BVD
CSB-EP409113BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Autophagy-related protein 8 (atg8) CSB-YP409114BVD
CSB-EP409114BVD
CSB-BP409114BVD
CSB-MP409114BVD
CSB-EP409114BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Golgi apparatus membrane protein tvp18 (tvp18), partial CSB-YP409115BVD1
CSB-EP409115BVD1
CSB-BP409115BVD1
CSB-MP409115BVD1
CSB-EP409115BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Serine/threonine-protein kinase atg1 (atg1), partial CSB-YP409116BVD
CSB-EP409116BVD
CSB-BP409116BVD
CSB-MP409116BVD
CSB-EP409116BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Nascent polypeptide-associated complex subunit beta (egd1) CSB-YP409117BVD
CSB-EP409117BVD
CSB-BP409117BVD
CSB-MP409117BVD
CSB-EP409117BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana tRNA (guanine-N (7)-)-methyltransferase CSB-YP409118BVD
CSB-EP409118BVD
CSB-BP409118BVD
CSB-MP409118BVD
CSB-EP409118BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Probable Xaa-Pro aminopeptidase pepP (pepP) CSB-YP409119BVD
CSB-EP409119BVD
CSB-BP409119BVD
CSB-MP409119BVD
CSB-EP409119BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana ATP-dependent RNA helicase dbp8 (dbp8), partial CSB-YP409120BVD
CSB-EP409120BVD
CSB-BP409120BVD
CSB-MP409120BVD
CSB-EP409120BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana ATP-dependent RNA helicase dbp9 (dbp9), partial CSB-YP409121BVD
CSB-EP409121BVD
CSB-BP409121BVD
CSB-MP409121BVD
CSB-EP409121BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP409122BVD1
CSB-EP409122BVD1
CSB-BP409122BVD1
CSB-MP409122BVD1
CSB-EP409122BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Arginine--tRNA ligase (argS), partial CSB-YP409123JAQ
CSB-EP409123JAQ
CSB-BP409123JAQ
CSB-MP409123JAQ
CSB-EP409123JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. UPF0102 protein mma_0204 (mma_0204) CSB-YP409124JAQ
CSB-EP409124JAQ
CSB-BP409124JAQ
CSB-MP409124JAQ
CSB-EP409124JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 30S ribosomal protein S9 (rpsI) CSB-YP409125JAQ
CSB-EP409125JAQ
CSB-BP409125JAQ
CSB-MP409125JAQ
CSB-EP409125JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP409126JAQ
CSB-EP409126JAQ
CSB-BP409126JAQ
CSB-MP409126JAQ
CSB-EP409126JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP409127JAQ
CSB-EP409127JAQ
CSB-BP409127JAQ
CSB-MP409127JAQ
CSB-EP409127JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP409128JAQ
CSB-EP409128JAQ
CSB-BP409128JAQ
CSB-MP409128JAQ
CSB-EP409128JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Alanine--tRNA ligase (alaS), partial CSB-YP409129JAQ
CSB-EP409129JAQ
CSB-BP409129JAQ
CSB-MP409129JAQ
CSB-EP409129JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP409130JAQ
CSB-EP409130JAQ
CSB-BP409130JAQ
CSB-MP409130JAQ
CSB-EP409130JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Glutamate--tRNA ligase (gltX) CSB-YP409131JAQ
CSB-EP409131JAQ
CSB-BP409131JAQ
CSB-MP409131JAQ
CSB-EP409131JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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