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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Ribosomal protein S6 modification protein (rimK) CSB-YP409472PZL
CSB-EP409472PZL
CSB-BP409472PZL
CSB-MP409472PZL
CSB-EP409472PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0149 protein PSPA7_5968 (PSPA7_5968) CSB-YP409473PZL
CSB-EP409473PZL
CSB-BP409473PZL
CSB-MP409473PZL
CSB-EP409473PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Xanthine phosphoribosyltransferase (xpt) CSB-YP409474PZL
CSB-EP409474PZL
CSB-BP409474PZL
CSB-MP409474PZL
CSB-EP409474PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP409475PZL
CSB-EP409475PZL
CSB-BP409475PZL
CSB-MP409475PZL
CSB-EP409475PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa ATP synthase epsilon chain (atpC) CSB-YP409476PZL
CSB-EP409476PZL
CSB-BP409476PZL
CSB-MP409476PZL
CSB-EP409476PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP409477PZL
CSB-EP409477PZL
CSB-BP409477PZL
CSB-MP409477PZL
CSB-EP409477PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis FO synthase subunit 1 (cofG) CSB-YP409478MNR
CSB-EP409478MNR
CSB-BP409478MNR
CSB-MP409478MNR
CSB-EP409478MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis tRNA (pseudouridine (54)-N (1))-methyltransferase CSB-YP409479MNR
CSB-EP409479MNR
CSB-BP409479MNR
CSB-MP409479MNR
CSB-EP409479MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis ATP phosphoribosyltransferase (hisG) CSB-YP409480MNR
CSB-EP409480MNR
CSB-BP409480MNR
CSB-MP409480MNR
CSB-EP409480MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP409481MNR
CSB-EP409481MNR
CSB-BP409481MNR
CSB-MP409481MNR
CSB-EP409481MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP409482MNR
CSB-EP409482MNR
CSB-BP409482MNR
CSB-MP409482MNR
CSB-EP409482MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase alpha chain (atpA), partial CSB-YP409483MNR
CSB-EP409483MNR
CSB-BP409483MNR
CSB-MP409483MNR
CSB-EP409483MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0219 protein MmarC7_0458 (MmarC7_0458) CSB-YP409484MNR
CSB-EP409484MNR
CSB-BP409484MNR
CSB-MP409484MNR
CSB-EP409484MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP409485MNR
CSB-EP409485MNR
CSB-BP409485MNR
CSB-MP409485MNR
CSB-EP409485MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S4P (rps4p) CSB-YP409486MNR
CSB-EP409486MNR
CSB-BP409486MNR
CSB-MP409486MNR
CSB-EP409486MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP409487MNR
CSB-EP409487MNR
CSB-BP409487MNR
CSB-MP409487MNR
CSB-EP409487MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Protein translation factor SUI1 homolog (MmarC7_0651) CSB-YP409488MNR
CSB-EP409488MNR
CSB-BP409488MNR
CSB-MP409488MNR
CSB-EP409488MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0210 protein MmarC7_0672 (MmarC7_0672) CSB-YP409489MNR
CSB-EP409489MNR
CSB-BP409489MNR
CSB-MP409489MNR
CSB-EP409489MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis GMP synthase [glutamine-hydrolyzing] subunit A (guaAA) CSB-YP409490MNR
CSB-EP409490MNR
CSB-BP409490MNR
CSB-MP409490MNR
CSB-EP409490MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Cobalt transport protein CbiN (cbiN), partial CSB-YP409491MNR1
CSB-EP409491MNR1
CSB-BP409491MNR1
CSB-MP409491MNR1
CSB-EP409491MNR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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