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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus aeolicus Proline--tRNA ligase (proS) CSB-YP409392MNO
CSB-EP409392MNO
CSB-BP409392MNO
CSB-MP409392MNO
CSB-EP409392MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable transaldolase (tal) CSB-YP409393MNO
CSB-EP409393MNO
CSB-BP409393MNO
CSB-MP409393MNO
CSB-EP409393MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Phosphoglucosamine mutase (glmM) CSB-YP409394MNO
CSB-EP409394MNO
CSB-BP409394MNO
CSB-MP409394MNO
CSB-EP409394MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus UPF0264 protein Maeo_0482 (Maeo_0482) CSB-YP409395MNO
CSB-EP409395MNO
CSB-BP409395MNO
CSB-MP409395MNO
CSB-EP409395MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP409396MNO
CSB-EP409396MNO
CSB-BP409396MNO
CSB-MP409396MNO
CSB-EP409396MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP409397MNO
CSB-EP409397MNO
CSB-BP409397MNO
CSB-MP409397MNO
CSB-EP409397MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Alanine--tRNA ligase (alaS), partial CSB-YP409398MNO
CSB-EP409398MNO
CSB-BP409398MNO
CSB-MP409398MNO
CSB-EP409398MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus DNA polymerase sliding clamp (pcn) CSB-YP409399MNO
CSB-EP409399MNO
CSB-BP409399MNO
CSB-MP409399MNO
CSB-EP409399MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Ribose-5-phosphate isomerase A (rpiA) CSB-YP409400MNO
CSB-EP409400MNO
CSB-BP409400MNO
CSB-MP409400MNO
CSB-EP409400MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Ribose 1,5-bisphosphate isomerase (Maeo_0798) CSB-YP409401MNO
CSB-EP409401MNO
CSB-BP409401MNO
CSB-MP409401MNO
CSB-EP409401MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable L-aspartate dehydrogenase (nadX) CSB-YP409402MNO
CSB-EP409402MNO
CSB-BP409402MNO
CSB-MP409402MNO
CSB-EP409402MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus GMP synthase [glutamine-hydrolyzing] subunit A (guaAA) CSB-YP409403MNO
CSB-EP409403MNO
CSB-BP409403MNO
CSB-MP409403MNO
CSB-EP409403MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Isoleucine--tRNA ligase (ileS), partial CSB-YP409404MNO
CSB-EP409404MNO
CSB-BP409404MNO
CSB-MP409404MNO
CSB-EP409404MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus L-tyrosine decarboxylase (mfnA) CSB-YP409405MNO
CSB-EP409405MNO
CSB-BP409405MNO
CSB-MP409405MNO
CSB-EP409405MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 30S ribosomal protein S4P (rps4p) CSB-YP409406MNO
CSB-EP409406MNO
CSB-BP409406MNO
CSB-MP409406MNO
CSB-EP409406MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L34e (rpl34e) CSB-YP409407MNO
CSB-EP409407MNO
CSB-BP409407MNO
CSB-MP409407MNO
CSB-EP409407MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Geranylgeranylglyceryl phosphate synthase (Maeo_1165) CSB-YP409408MNO
CSB-EP409408MNO
CSB-BP409408MNO
CSB-MP409408MNO
CSB-EP409408MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Signal recognition 54 kDa protein (srp54) CSB-YP409409MNO
CSB-EP409409MNO
CSB-BP409409MNO
CSB-MP409409MNO
CSB-EP409409MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP409410MNO
CSB-EP409410MNO
CSB-BP409410MNO
CSB-MP409410MNO
CSB-EP409410MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Prefoldin subunit alpha (pfdA) CSB-YP409411MNO
CSB-EP409411MNO
CSB-BP409411MNO
CSB-MP409411MNO
CSB-EP409411MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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