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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Chorismate synthase (aroC) CSB-YP409652STM
CSB-EP409652STM
CSB-BP409652STM
CSB-MP409652STM
CSB-EP409652STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Flagellar P-ring protein (flgI) CSB-YP409653STM
CSB-EP409653STM
CSB-BP409653STM
CSB-MP409653STM
CSB-EP409653STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP409654STM
CSB-EP409654STM
CSB-BP409654STM
CSB-MP409654STM
CSB-EP409654STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Alanine--tRNA ligase (alaS), partial CSB-YP409655STM
CSB-EP409655STM
CSB-BP409655STM
CSB-MP409655STM
CSB-EP409655STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP409656STM
CSB-EP409656STM
CSB-BP409656STM
CSB-MP409656STM
CSB-EP409656STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Deoxyribose-phosphate aldolase (deoC) CSB-YP409657STM
CSB-EP409657STM
CSB-BP409657STM
CSB-MP409657STM
CSB-EP409657STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphoglucosamine mutase 1 (glmM1) CSB-YP409658STM
CSB-EP409658STM
CSB-BP409658STM
CSB-MP409658STM
CSB-EP409658STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0250 protein Shew185_3322 (Shew185_3322) CSB-YP409659STM
CSB-EP409659STM
CSB-BP409659STM
CSB-MP409659STM
CSB-EP409659STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA polymerase IV (dinB) CSB-YP409660STM
CSB-EP409660STM
CSB-BP409660STM
CSB-MP409660STM
CSB-EP409660STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP409661STM
CSB-EP409661STM
CSB-BP409661STM
CSB-MP409661STM
CSB-EP409661STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glutamyl-tRNA reductase (hemA) CSB-YP409662STM
CSB-EP409662STM
CSB-BP409662STM
CSB-MP409662STM
CSB-EP409662STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Arginine repressor (argR) CSB-YP409663STM
CSB-EP409663STM
CSB-BP409663STM
CSB-MP409663STM
CSB-EP409663STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Large-conductance mechanosensitive channel (mscL), partial CSB-YP409664STM1
CSB-EP409664STM1
CSB-BP409664STM1
CSB-MP409664STM1
CSB-EP409664STM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal protein L11 methyltransferase (prmA) CSB-YP409665STM
CSB-EP409665STM
CSB-BP409665STM
CSB-MP409665STM
CSB-EP409665STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0102 protein Shew185_4070 (Shew185_4070) CSB-YP409666STM
CSB-EP409666STM
CSB-BP409666STM
CSB-MP409666STM
CSB-EP409666STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica CinA-like protein (Shew185_4098) CSB-YP409667STM
CSB-EP409667STM
CSB-BP409667STM
CSB-MP409667STM
CSB-EP409667STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP409668STM
CSB-EP409668STM
CSB-BP409668STM
CSB-MP409668STM
CSB-EP409668STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase epsilon chain (atpC) CSB-YP409669STM
CSB-EP409669STM
CSB-BP409669STM
CSB-MP409669STM
CSB-EP409669STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP409670STM
CSB-EP409670STM
CSB-BP409670STM
CSB-MP409670STM
CSB-EP409670STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi UPF0133 protein Oant_0022 (Oant_0022) CSB-YP409671ODH
CSB-EP409671ODH
CSB-BP409671ODH
CSB-MP409671ODH
CSB-EP409671ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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