Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kineococcus radiotolerans DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP410453KAW
CSB-EP410453KAW
CSB-BP410453KAW
CSB-MP410453KAW
CSB-EP410453KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 30S ribosomal protein S19 (rpsS) CSB-YP410454KAW
CSB-EP410454KAW
CSB-BP410454KAW
CSB-MP410454KAW
CSB-EP410454KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L5 (rplE) CSB-YP410455KAW
CSB-EP410455KAW
CSB-BP410455KAW
CSB-MP410455KAW
CSB-EP410455KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L36 2 (rpmJ2) CSB-YP410456KAW
CSB-EP410456KAW
CSB-BP410456KAW
CSB-MP410456KAW
CSB-EP410456KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Phosphoglucosamine mutase (glmM) CSB-YP410457KAW
CSB-EP410457KAW
CSB-BP410457KAW
CSB-MP410457KAW
CSB-EP410457KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Hydroxyethylthiazole kinase (thiM) CSB-YP410458KAW
CSB-EP410458KAW
CSB-BP410458KAW
CSB-MP410458KAW
CSB-EP410458KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP410459KAW
CSB-EP410459KAW
CSB-BP410459KAW
CSB-MP410459KAW
CSB-EP410459KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP410460KAW
CSB-EP410460KAW
CSB-BP410460KAW
CSB-MP410460KAW
CSB-EP410460KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L32 (rpmF) CSB-YP410461KAW
CSB-EP410461KAW
CSB-BP410461KAW
CSB-MP410461KAW
CSB-EP410461KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP410462KAW
CSB-EP410462KAW
CSB-BP410462KAW
CSB-MP410462KAW
CSB-EP410462KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Proteasome-associated ATPase (arc), partial CSB-YP410463KAW
CSB-EP410463KAW
CSB-BP410463KAW
CSB-MP410463KAW
CSB-EP410463KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Putative sporulation transcription regulator WhiA (whiA) CSB-YP410464KAW
CSB-EP410464KAW
CSB-BP410464KAW
CSB-MP410464KAW
CSB-EP410464KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP410465KAW
CSB-EP410465KAW
CSB-BP410465KAW
CSB-MP410465KAW
CSB-EP410465KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Probable transcriptional regulatory protein Krad_3057 (Krad_3057) CSB-YP410466KAW
CSB-EP410466KAW
CSB-BP410466KAW
CSB-MP410466KAW
CSB-EP410466KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L35 (rpmI) CSB-YP410467KAW
CSB-EP410467KAW
CSB-BP410467KAW
CSB-MP410467KAW
CSB-EP410467KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Dihydroorotate dehydrogenase (quinone) CSB-YP410468KAW
CSB-EP410468KAW
CSB-BP410468KAW
CSB-MP410468KAW
CSB-EP410468KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Leucine--tRNA ligase (leuS), partial CSB-YP410469KAW
CSB-EP410469KAW
CSB-BP410469KAW
CSB-MP410469KAW
CSB-EP410469KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 60 kDa chaperonin 2 (groL2), partial CSB-YP410470KAW
CSB-EP410470KAW
CSB-BP410470KAW
CSB-MP410470KAW
CSB-EP410470KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Inositol 2-dehydrogenase (iolG) CSB-YP410471KAW
CSB-EP410471KAW
CSB-BP410471KAW
CSB-MP410471KAW
CSB-EP410471KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Sulfurtransferase TusA homolog (tusA) CSB-YP410472STM
CSB-EP410472STM
CSB-BP410472STM
CSB-MP410472STM
CSB-EP410472STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>