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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus aeolicus Biotin synthase (bioB) CSB-YP410253MNO
CSB-EP410253MNO
CSB-BP410253MNO
CSB-MP410253MNO
CSB-EP410253MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Histidinol-phosphate aminotransferase (hisC) CSB-YP410254MNO
CSB-EP410254MNO
CSB-BP410254MNO
CSB-MP410254MNO
CSB-EP410254MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L37e (rpl37e) CSB-YP410255MNO
CSB-EP410255MNO
CSB-BP410255MNO
CSB-MP410255MNO
CSB-EP410255MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable thymidylate kinase (tmk) CSB-YP410256MNO
CSB-EP410256MNO
CSB-BP410256MNO
CSB-MP410256MNO
CSB-EP410256MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Shikimate dehydrogenase (aroE) CSB-YP410257MNO
CSB-EP410257MNO
CSB-BP410257MNO
CSB-MP410257MNO
CSB-EP410257MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Putative thymidine phosphorylase (Maeo_0618), partial CSB-YP410258MNO
CSB-EP410258MNO
CSB-BP410258MNO
CSB-MP410258MNO
CSB-EP410258MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Ribosome biogenesis protein Nop10 (Maeo_0704) CSB-YP410259MNO
CSB-EP410259MNO
CSB-BP410259MNO
CSB-MP410259MNO
CSB-EP410259MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP410260MNO
CSB-EP410260MNO
CSB-BP410260MNO
CSB-MP410260MNO
CSB-EP410260MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 30S ribosomal protein S12P (rps12p) CSB-YP410261MNO
CSB-EP410261MNO
CSB-BP410261MNO
CSB-MP410261MNO
CSB-EP410261MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus UPF0235 protein Maeo_0841 (Maeo_0841) CSB-YP410262MNO
CSB-EP410262MNO
CSB-BP410262MNO
CSB-MP410262MNO
CSB-EP410262MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus FO synthase subunit 1 (cofG) CSB-YP410263MNO
CSB-EP410263MNO
CSB-BP410263MNO
CSB-MP410263MNO
CSB-EP410263MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L39e (rpl39e) CSB-YP410264MNO
CSB-EP410264MNO
CSB-BP410264MNO
CSB-MP410264MNO
CSB-EP410264MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Digeranylgeranylglyceryl phosphate synthase (Maeo_1040), partial CSB-YP410265MNO
CSB-EP410265MNO
CSB-BP410265MNO
CSB-MP410265MNO
CSB-EP410265MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Arginine--tRNA ligase (argS) CSB-YP410266MNO
CSB-EP410266MNO
CSB-BP410266MNO
CSB-MP410266MNO
CSB-EP410266MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Transcription factor E (tfe) CSB-YP410267MNO
CSB-EP410267MNO
CSB-BP410267MNO
CSB-MP410267MNO
CSB-EP410267MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Threonine--tRNA ligase (thrS) CSB-YP410268MNO
CSB-EP410268MNO
CSB-BP410268MNO
CSB-MP410268MNO
CSB-EP410268MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Tetrahydromethanopterin S-methyltransferase subunit E (mtrE), partial CSB-YP410269MNO1
CSB-EP410269MNO1
CSB-BP410269MNO1
CSB-MP410269MNO1
CSB-EP410269MNO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP410270MNO
CSB-EP410270MNO
CSB-BP410270MNO
CSB-MP410270MNO
CSB-EP410270MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L22P (rpl22p) CSB-YP410271MNO
CSB-EP410271MNO
CSB-BP410271MNO
CSB-MP410271MNO
CSB-EP410271MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 30S ribosomal protein S8P (rps8p) CSB-YP410272MNO
CSB-EP410272MNO
CSB-BP410272MNO
CSB-MP410272MNO
CSB-EP410272MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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