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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP410513STM
CSB-EP410513STM
CSB-BP410513STM
CSB-MP410513STM
CSB-EP410513STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP410514STM
CSB-EP410514STM
CSB-BP410514STM
CSB-MP410514STM
CSB-EP410514STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP410515ODH
CSB-EP410515ODH
CSB-BP410515ODH
CSB-MP410515ODH
CSB-EP410515ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Integration host factor subunit beta (ihfB) CSB-YP410516ODH
CSB-EP410516ODH
CSB-BP410516ODH
CSB-MP410516ODH
CSB-EP410516ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP410517ODH
CSB-EP410517ODH
CSB-BP410517ODH
CSB-MP410517ODH
CSB-EP410517ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Urease accessory protein UreG (ureG) CSB-YP410518ODH
CSB-EP410518ODH
CSB-BP410518ODH
CSB-MP410518ODH
CSB-EP410518ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP410519ODH
CSB-EP410519ODH
CSB-BP410519ODH
CSB-MP410519ODH
CSB-EP410519ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP410520ODH
CSB-EP410520ODH
CSB-BP410520ODH
CSB-MP410520ODH
CSB-EP410520ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi tRNA pseudouridine synthase B (truB) CSB-YP410521ODH
CSB-EP410521ODH
CSB-BP410521ODH
CSB-MP410521ODH
CSB-EP410521ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Tryptophan synthase alpha chain (trpA) CSB-YP410522ODH
CSB-EP410522ODH
CSB-BP410522ODH
CSB-MP410522ODH
CSB-EP410522ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP410523ODH
CSB-EP410523ODH
CSB-BP410523ODH
CSB-MP410523ODH
CSB-EP410523ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi tRNA modification GTPase MnmE (mnmE) CSB-YP410524ODH
CSB-EP410524ODH
CSB-BP410524ODH
CSB-MP410524ODH
CSB-EP410524ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Ribosome maturation factor RimM (rimM) CSB-YP410525ODH
CSB-EP410525ODH
CSB-BP410525ODH
CSB-MP410525ODH
CSB-EP410525ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi 7-cyano-7-deazaguanine synthase (queC) CSB-YP410526ODH
CSB-EP410526ODH
CSB-BP410526ODH
CSB-MP410526ODH
CSB-EP410526ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Glutamate 5-kinase (proB) CSB-YP410527ODH
CSB-EP410527ODH
CSB-BP410527ODH
CSB-MP410527ODH
CSB-EP410527ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi ATP synthase gamma chain (atpG) CSB-YP410528ODH
CSB-EP410528ODH
CSB-BP410528ODH
CSB-MP410528ODH
CSB-EP410528ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Probable transcriptional regulatory protein Oant_1200 (Oant_1200) CSB-YP410529ODH
CSB-EP410529ODH
CSB-BP410529ODH
CSB-MP410529ODH
CSB-EP410529ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Adenylosuccinate synthetase (purA) CSB-YP410530ODH
CSB-EP410530ODH
CSB-BP410530ODH
CSB-MP410530ODH
CSB-EP410530ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Elongation factor 4 (lepA), partial CSB-YP410531ODH
CSB-EP410531ODH
CSB-BP410531ODH
CSB-MP410531ODH
CSB-EP410531ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Ribonuclease P protein component (rnpA) CSB-YP410532ODH
CSB-EP410532ODH
CSB-BP410532ODH
CSB-MP410532ODH
CSB-EP410532ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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