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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP412114STM
CSB-EP412114STM
CSB-BP412114STM
CSB-MP412114STM
CSB-EP412114STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S16 (rpsP) CSB-YP412115STM
CSB-EP412115STM
CSB-BP412115STM
CSB-MP412115STM
CSB-EP412115STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP412116STM
CSB-EP412116STM
CSB-BP412116STM
CSB-MP412116STM
CSB-EP412116STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein syd (syd) CSB-YP412117STM
CSB-EP412117STM
CSB-BP412117STM
CSB-MP412117STM
CSB-EP412117STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Putative reductase Shew185_1599 (Shew185_1599) CSB-YP412118STM
CSB-EP412118STM
CSB-BP412118STM
CSB-MP412118STM
CSB-EP412118STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP412119STM
CSB-EP412119STM
CSB-BP412119STM
CSB-MP412119STM
CSB-EP412119STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Biosynthetic arginine decarboxylase (speA), partial CSB-YP412120STM
CSB-EP412120STM
CSB-BP412120STM
CSB-MP412120STM
CSB-EP412120STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Integration host factor subunit alpha (ihfA) CSB-YP412121STM
CSB-EP412121STM
CSB-BP412121STM
CSB-MP412121STM
CSB-EP412121STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribonuclease H (rnhA) CSB-YP412122STM
CSB-EP412122STM
CSB-BP412122STM
CSB-MP412122STM
CSB-EP412122STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Succinylglutamate desuccinylase (astE) CSB-YP412123STM
CSB-EP412123STM
CSB-BP412123STM
CSB-MP412123STM
CSB-EP412123STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Integration host factor subunit beta (ihfB) CSB-YP412124STM
CSB-EP412124STM
CSB-BP412124STM
CSB-MP412124STM
CSB-EP412124STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Aspartate--tRNA ligase (aspS), partial CSB-YP412125STM
CSB-EP412125STM
CSB-BP412125STM
CSB-MP412125STM
CSB-EP412125STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP412126STM
CSB-EP412126STM
CSB-BP412126STM
CSB-MP412126STM
CSB-EP412126STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP phosphoribosyltransferase (hisG) CSB-YP412127STM
CSB-EP412127STM
CSB-BP412127STM
CSB-MP412127STM
CSB-EP412127STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Methionine--tRNA ligase (metG), partial CSB-YP412128STM
CSB-EP412128STM
CSB-BP412128STM
CSB-MP412128STM
CSB-EP412128STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Dihydrodipicolinate synthase (dapA) CSB-YP412129STM
CSB-EP412129STM
CSB-BP412129STM
CSB-MP412129STM
CSB-EP412129STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Recombination protein RecR (recR) CSB-YP412130STM
CSB-EP412130STM
CSB-BP412130STM
CSB-MP412130STM
CSB-EP412130STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP412131STM
CSB-EP412131STM
CSB-BP412131STM
CSB-MP412131STM
CSB-EP412131STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA (guanine-N (7)-)-methyltransferase CSB-YP412132STM
CSB-EP412132STM
CSB-BP412132STM
CSB-MP412132STM
CSB-EP412132STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP412133STM
CSB-EP412133STM
CSB-BP412133STM
CSB-MP412133STM
CSB-EP412133STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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