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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sinorhizobium medicae Putative Holliday junction resolvase (Smed_0941) CSB-YP410933STV
CSB-EP410933STV
CSB-BP410933STV
CSB-MP410933STV
CSB-EP410933STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP410934STV
CSB-EP410934STV
CSB-BP410934STV
CSB-MP410934STV
CSB-EP410934STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L7/L12 (rplL) CSB-YP410935STV
CSB-EP410935STV
CSB-BP410935STV
CSB-MP410935STV
CSB-EP410935STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 30S ribosomal protein S12 (rpsL) CSB-YP410936STV
CSB-EP410936STV
CSB-BP410936STV
CSB-MP410936STV
CSB-EP410936STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L3 (rplC) CSB-YP410937STV
CSB-EP410937STV
CSB-BP410937STV
CSB-MP410937STV
CSB-EP410937STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L2 (rplB) CSB-YP410938STV
CSB-EP410938STV
CSB-BP410938STV
CSB-MP410938STV
CSB-EP410938STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 50S ribosomal protein L29 (rpmC) CSB-YP410939STV
CSB-EP410939STV
CSB-BP410939STV
CSB-MP410939STV
CSB-EP410939STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 30S ribosomal protein S8 (rpsH) CSB-YP410940STV
CSB-EP410940STV
CSB-BP410940STV
CSB-MP410940STV
CSB-EP410940STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP410941STV
CSB-EP410941STV
CSB-BP410941STV
CSB-MP410941STV
CSB-EP410941STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Enolase (eno) CSB-YP410942STV
CSB-EP410942STV
CSB-BP410942STV
CSB-MP410942STV
CSB-EP410942STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP410943STV
CSB-EP410943STV
CSB-BP410943STV
CSB-MP410943STV
CSB-EP410943STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Serine--tRNA ligase (serS) CSB-YP410944STV
CSB-EP410944STV
CSB-BP410944STV
CSB-MP410944STV
CSB-EP410944STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae 60 kDa chaperonin 3 (groL3), partial CSB-YP410945STV
CSB-EP410945STV
CSB-BP410945STV
CSB-MP410945STV
CSB-EP410945STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Anthranilate phosphoribosyltransferase (trpD) CSB-YP410946STV
CSB-EP410946STV
CSB-BP410946STV
CSB-MP410946STV
CSB-EP410946STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Alanine--tRNA ligase (alaS) CSB-YP410947STV
CSB-EP410947STV
CSB-BP410947STV
CSB-MP410947STV
CSB-EP410947STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Cobalamin synthase (cobS), partial CSB-YP410948STV1
CSB-EP410948STV1
CSB-BP410948STV1
CSB-MP410948STV1
CSB-EP410948STV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae D-alanine--D-alanine ligase (ddl) CSB-YP410949STV
CSB-EP410949STV
CSB-BP410949STV
CSB-MP410949STV
CSB-EP410949STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Probable D-serine dehydratase (dsdA) CSB-YP410950STV
CSB-EP410950STV
CSB-BP410950STV
CSB-MP410950STV
CSB-EP410950STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Deoxyribose-phosphate aldolase (deoC) CSB-YP410951STV
CSB-EP410951STV
CSB-BP410951STV
CSB-MP410951STV
CSB-EP410951STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinorhizobium medicae Adenylosuccinate synthetase (purA) CSB-YP410952STV
CSB-EP410952STV
CSB-BP410952STV
CSB-MP410952STV
CSB-EP410952STV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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