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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus vannielii Enolase (eno) CSB-YP411873MNS
CSB-EP411873MNS
CSB-BP411873MNS
CSB-MP411873MNS
CSB-EP411873MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Dihydrodipicolinate synthase (dapA) CSB-YP411874MNS
CSB-EP411874MNS
CSB-BP411874MNS
CSB-MP411874MNS
CSB-EP411874MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP411875MNS
CSB-EP411875MNS
CSB-BP411875MNS
CSB-MP411875MNS
CSB-EP411875MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase (Mevan_1506) CSB-YP411876MNS
CSB-EP411876MNS
CSB-BP411876MNS
CSB-MP411876MNS
CSB-EP411876MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii UDP-N-acetyl-D-mannosamine dehydrogenase (wecC) CSB-YP411877MNS
CSB-EP411877MNS
CSB-BP411877MNS
CSB-MP411877MNS
CSB-EP411877MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Aspartate--tRNA ligase (aspS) CSB-YP411878MNO
CSB-EP411878MNO
CSB-BP411878MNO
CSB-MP411878MNO
CSB-EP411878MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP411879MNO
CSB-EP411879MNO
CSB-BP411879MNO
CSB-MP411879MNO
CSB-EP411879MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus V-type ATP synthase beta chain (atpB) CSB-YP411880MNO
CSB-EP411880MNO
CSB-BP411880MNO
CSB-MP411880MNO
CSB-EP411880MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein LX (rplX) CSB-YP411881MNO
CSB-EP411881MNO
CSB-BP411881MNO
CSB-MP411881MNO
CSB-EP411881MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP411882MNO
CSB-EP411882MNO
CSB-BP411882MNO
CSB-MP411882MNO
CSB-EP411882MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Acidic ribosomal protein P0 homolog (rplP0) CSB-YP411883MNO
CSB-EP411883MNO
CSB-BP411883MNO
CSB-MP411883MNO
CSB-EP411883MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase (Maeo_0316) CSB-YP411884MNO
CSB-EP411884MNO
CSB-BP411884MNO
CSB-MP411884MNO
CSB-EP411884MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP411885MNO
CSB-EP411885MNO
CSB-BP411885MNO
CSB-MP411885MNO
CSB-EP411885MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Translation initiation factor 1A (eIF1A) CSB-YP411886MNO
CSB-EP411886MNO
CSB-BP411886MNO
CSB-MP411886MNO
CSB-EP411886MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Bifunctional protein GlmU (Maeo_0642) CSB-YP411887MNO
CSB-EP411887MNO
CSB-BP411887MNO
CSB-MP411887MNO
CSB-EP411887MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus tRNA (pseudouridine (54)-N (1))-methyltransferase CSB-YP411888MNO
CSB-EP411888MNO
CSB-BP411888MNO
CSB-MP411888MNO
CSB-EP411888MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable cobalamin biosynthesis protein CobD (cobD), partial CSB-YP411889MNO1
CSB-EP411889MNO1
CSB-BP411889MNO1
CSB-MP411889MNO1
CSB-EP411889MNO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Probable tRNA pseudouridine synthase D (truD) CSB-YP411890MNO
CSB-EP411890MNO
CSB-BP411890MNO
CSB-MP411890MNO
CSB-EP411890MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 30S ribosomal protein S7P (rps7p) CSB-YP411891MNO
CSB-EP411891MNO
CSB-BP411891MNO
CSB-MP411891MNO
CSB-EP411891MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus 50S ribosomal protein L23P (rpl23p) CSB-YP411892MNO
CSB-EP411892MNO
CSB-BP411892MNO
CSB-MP411892MNO
CSB-EP411892MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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