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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Marinomonas sp. 50S ribosomal protein L14 (rplN) CSB-YP412073MNJ
CSB-EP412073MNJ
CSB-BP412073MNJ
CSB-MP412073MNJ
CSB-EP412073MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L10 (rplJ) CSB-YP412074MNJ
CSB-EP412074MNJ
CSB-BP412074MNJ
CSB-MP412074MNJ
CSB-EP412074MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. tRNA (uracil (54)-C (5))-methyltransferase CSB-YP412075MNJ
CSB-EP412075MNJ
CSB-BP412075MNJ
CSB-MP412075MNJ
CSB-EP412075MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. ATP synthase subunit beta 2 (atpD2) CSB-YP412076MNJ
CSB-EP412076MNJ
CSB-BP412076MNJ
CSB-MP412076MNJ
CSB-EP412076MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP412077MNJ
CSB-EP412077MNJ
CSB-BP412077MNJ
CSB-MP412077MNJ
CSB-EP412077MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans DNA replication and repair protein recF (recF) CSB-YP412078KAW
CSB-EP412078KAW
CSB-BP412078KAW
CSB-MP412078KAW
CSB-EP412078KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP412079KAW
CSB-EP412079KAW
CSB-BP412079KAW
CSB-MP412079KAW
CSB-EP412079KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP412080KAW
CSB-EP412080KAW
CSB-BP412080KAW
CSB-MP412080KAW
CSB-EP412080KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 30S ribosomal protein S7 (rpsG) CSB-YP412081KAW
CSB-EP412081KAW
CSB-BP412081KAW
CSB-MP412081KAW
CSB-EP412081KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 30S ribosomal protein S14 type Z (rpsZ) CSB-YP412082KAW
CSB-EP412082KAW
CSB-BP412082KAW
CSB-MP412082KAW
CSB-EP412082KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L13 (rplM) CSB-YP412083KAW
CSB-EP412083KAW
CSB-BP412083KAW
CSB-MP412083KAW
CSB-EP412083KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Potassium-transporting ATPase C chain (kdpC), partial CSB-YP412084KAW1
CSB-EP412084KAW1
CSB-BP412084KAW1
CSB-MP412084KAW1
CSB-EP412084KAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP412085KAW
CSB-EP412085KAW
CSB-BP412085KAW
CSB-MP412085KAW
CSB-EP412085KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Arginine--tRNA ligase (argS), partial CSB-YP412086KAW
CSB-EP412086KAW
CSB-BP412086KAW
CSB-MP412086KAW
CSB-EP412086KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans DNA ligase (ligA), partial CSB-YP412087KAW
CSB-EP412087KAW
CSB-BP412087KAW
CSB-MP412087KAW
CSB-EP412087KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans D-alanine--D-alanine ligase (ddl) CSB-YP412088KAW
CSB-EP412088KAW
CSB-BP412088KAW
CSB-MP412088KAW
CSB-EP412088KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Dihydrodipicolinate synthase (dapA) CSB-YP412089KAW
CSB-EP412089KAW
CSB-BP412089KAW
CSB-MP412089KAW
CSB-EP412089KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Na (+)/H (+) antiporter nhaA 2, partial CSB-YP412090KAW
CSB-EP412090KAW
CSB-BP412090KAW
CSB-MP412090KAW
CSB-EP412090KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP412091KAW
CSB-EP412091KAW
CSB-BP412091KAW
CSB-MP412091KAW
CSB-EP412091KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L20 (rplT) CSB-YP412092KAW
CSB-EP412092KAW
CSB-BP412092KAW
CSB-MP412092KAW
CSB-EP412092KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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