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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa Chaperone protein DnaK (dnaK), partial CSB-YP411954PZL
CSB-EP411954PZL
CSB-BP411954PZL
CSB-MP411954PZL
CSB-EP411954PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosomal protein L11 methyltransferase (prmA) CSB-YP411955PZL
CSB-EP411955PZL
CSB-BP411955PZL
CSB-MP411955PZL
CSB-EP411955PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Urease accessory protein UreG (ureG) CSB-YP411956PZL
CSB-EP411956PZL
CSB-BP411956PZL
CSB-MP411956PZL
CSB-EP411956PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA mismatch repair protein mutL (mutL), partial CSB-YP411957PZL
CSB-EP411957PZL
CSB-BP411957PZL
CSB-MP411957PZL
CSB-EP411957PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP411958PZL
CSB-EP411958PZL
CSB-BP411958PZL
CSB-MP411958PZL
CSB-EP411958PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein-export protein secB (secB) CSB-YP411959PZL
CSB-EP411959PZL
CSB-BP411959PZL
CSB-MP411959PZL
CSB-EP411959PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable Fe (2+)-trafficking protein CSB-YP411960PZL
CSB-EP411960PZL
CSB-BP411960PZL
CSB-MP411960PZL
CSB-EP411960PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Diaminopimelate epimerase (dapF) CSB-YP411961PZL
CSB-EP411961PZL
CSB-BP411961PZL
CSB-MP411961PZL
CSB-EP411961PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribonuclease PH (rph) CSB-YP411962PZL
CSB-EP411962PZL
CSB-BP411962PZL
CSB-MP411962PZL
CSB-EP411962PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Pyridoxamine kinase (pdxY) CSB-YP411963PZL
CSB-EP411963PZL
CSB-BP411963PZL
CSB-MP411963PZL
CSB-EP411963PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP411964MNR
CSB-EP411964MNR
CSB-BP411964MNR
CSB-MP411964MNR
CSB-EP411964MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease P protein component 4 (rnp4) CSB-YP411965MNR
CSB-EP411965MNR
CSB-BP411965MNR
CSB-MP411965MNR
CSB-EP411965MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis F420-0:gamma-glutamyl ligase (cofE) CSB-YP411966MNR
CSB-EP411966MNR
CSB-BP411966MNR
CSB-MP411966MNR
CSB-EP411966MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP411967MNR
CSB-EP411967MNR
CSB-BP411967MNR
CSB-MP411967MNR
CSB-EP411967MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5'-nucleotidase surE (surE) CSB-YP411968MNR
CSB-EP411968MNR
CSB-BP411968MNR
CSB-MP411968MNR
CSB-EP411968MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Peptide chain release factor subunit 1 (prf1) CSB-YP411969MNR
CSB-EP411969MNR
CSB-BP411969MNR
CSB-MP411969MNR
CSB-EP411969MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose-5-phosphate isomerase A (rpiA) CSB-YP411970MNR
CSB-EP411970MNR
CSB-BP411970MNR
CSB-MP411970MNR
CSB-EP411970MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 2 subunit gamma (eIF2G) CSB-YP411971MNR
CSB-EP411971MNR
CSB-BP411971MNR
CSB-MP411971MNR
CSB-EP411971MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis O-phospho-L-seryl-tRNA:Cys-tRNA synthase (MmarC7_0486) CSB-YP411972MNR
CSB-EP411972MNR
CSB-BP411972MNR
CSB-MP411972MNR
CSB-EP411972MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable transaldolase (tal) CSB-YP411973MNR
CSB-EP411973MNR
CSB-BP411973MNR
CSB-MP411973MNR
CSB-EP411973MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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