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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP414414VEA
CSB-EP414414VEA
CSB-BP414414VEA
CSB-MP414414VEA
CSB-EP414414VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Aspartate carbamoyltransferase (pyrB) CSB-YP414415VEA
CSB-EP414415VEA
CSB-BP414415VEA
CSB-MP414415VEA
CSB-EP414415VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0758 protein VIBHAR_00653 (VIBHAR_00653) CSB-YP414416VEA
CSB-EP414416VEA
CSB-BP414416VEA
CSB-MP414416VEA
CSB-EP414416VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Outer-membrane lipoprotein carrier protein (lolA) CSB-YP414417VEA
CSB-EP414417VEA
CSB-BP414417VEA
CSB-MP414417VEA
CSB-EP414417VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 3-dehydroquinate synthase (aroB) CSB-YP414418VEA
CSB-EP414418VEA
CSB-BP414418VEA
CSB-MP414418VEA
CSB-EP414418VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Bifunctional protein FolD (folD) CSB-YP414419VEA
CSB-EP414419VEA
CSB-BP414419VEA
CSB-MP414419VEA
CSB-EP414419VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribose-5-phosphate isomerase A (rpiA) CSB-YP414420VEA
CSB-EP414420VEA
CSB-BP414420VEA
CSB-MP414420VEA
CSB-EP414420VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi DNA mismatch repair protein MutS (mutS), partial CSB-YP414421VEA
CSB-EP414421VEA
CSB-BP414421VEA
CSB-MP414421VEA
CSB-EP414421VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP414422VEA
CSB-EP414422VEA
CSB-BP414422VEA
CSB-MP414422VEA
CSB-EP414422VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Recombination protein RecR (recR) CSB-YP414423VEA
CSB-EP414423VEA
CSB-BP414423VEA
CSB-MP414423VEA
CSB-EP414423VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP414424VEA
CSB-EP414424VEA
CSB-BP414424VEA
CSB-MP414424VEA
CSB-EP414424VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphopentomutase (deoB) CSB-YP414425VEA
CSB-EP414425VEA
CSB-BP414425VEA
CSB-MP414425VEA
CSB-EP414425VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0061 protein VIBHAR_01411 (VIBHAR_01411) CSB-YP414426VEA
CSB-EP414426VEA
CSB-BP414426VEA
CSB-MP414426VEA
CSB-EP414426VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Uridine kinase (udk) CSB-YP414427VEA
CSB-EP414427VEA
CSB-BP414427VEA
CSB-MP414427VEA
CSB-EP414427VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0299 membrane protein VIBHAR_02118 (VIBHAR_02118) CSB-YP414428VEA
CSB-EP414428VEA
CSB-BP414428VEA
CSB-MP414428VEA
CSB-EP414428VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP414429VEA
CSB-EP414429VEA
CSB-BP414429VEA
CSB-MP414429VEA
CSB-EP414429VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP414430VEA
CSB-EP414430VEA
CSB-BP414430VEA
CSB-MP414430VEA
CSB-EP414430VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L18 (rplR) CSB-YP414431VEA
CSB-EP414431VEA
CSB-BP414431VEA
CSB-MP414431VEA
CSB-EP414431VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L4 (rplD) CSB-YP414432VEA
CSB-EP414432VEA
CSB-BP414432VEA
CSB-MP414432VEA
CSB-EP414432VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP414433VEA
CSB-EP414433VEA
CSB-BP414433VEA
CSB-MP414433VEA
CSB-EP414433VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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