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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus subsp. cytotoxis Putative septation protein spoVG (spoVG) CSB-YP415414BOK
CSB-EP415414BOK
CSB-BP415414BOK
CSB-MP415414BOK
CSB-EP415414BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 30S ribosomal protein S12 (rpsL) CSB-YP415415BOK
CSB-EP415415BOK
CSB-BP415415BOK
CSB-MP415415BOK
CSB-EP415415BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L4 (rplD) CSB-YP415416BOK
CSB-EP415416BOK
CSB-BP415416BOK
CSB-MP415416BOK
CSB-EP415416BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L14 (rplN) CSB-YP415417BOK
CSB-EP415417BOK
CSB-BP415417BOK
CSB-MP415417BOK
CSB-EP415417BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L36 (rpmJ) CSB-YP415418BOK
CSB-EP415418BOK
CSB-BP415418BOK
CSB-MP415418BOK
CSB-EP415418BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phosphoglucosamine mutase (glmM) CSB-YP415419BOK
CSB-EP415419BOK
CSB-BP415419BOK
CSB-MP415419BOK
CSB-EP415419BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP415420BOK
CSB-EP415420BOK
CSB-BP415420BOK
CSB-MP415420BOK
CSB-EP415420BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP415421BOK
CSB-EP415421BOK
CSB-BP415421BOK
CSB-MP415421BOK
CSB-EP415421BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0435 protein Bcer98_0391 (Bcer98_0391) CSB-YP415422BOK
CSB-EP415422BOK
CSB-BP415422BOK
CSB-MP415422BOK
CSB-EP415422BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Probable transcriptional regulatory protein Bcer98_0465 (Bcer98_0465) CSB-YP415423BOK
CSB-EP415423BOK
CSB-BP415423BOK
CSB-MP415423BOK
CSB-EP415423BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0342 protein Bcer98_0695 (Bcer98_0695) CSB-YP415424BOK
CSB-EP415424BOK
CSB-BP415424BOK
CSB-MP415424BOK
CSB-EP415424BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Lactate utilization protein A (lutA) CSB-YP415425BOK
CSB-EP415425BOK
CSB-BP415425BOK
CSB-MP415425BOK
CSB-EP415425BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phosphonoacetaldehyde hydrolase (phnX) CSB-YP415426BOK
CSB-EP415426BOK
CSB-BP415426BOK
CSB-MP415426BOK
CSB-EP415426BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 2-isopropylmalate synthase (leuA), partial CSB-YP415427BOK
CSB-EP415427BOK
CSB-BP415427BOK
CSB-MP415427BOK
CSB-EP415427BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP415428BOK
CSB-EP415428BOK
CSB-BP415428BOK
CSB-MP415428BOK
CSB-EP415428BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Dihydrodipicolinate reductase (dapB) CSB-YP415429BOK
CSB-EP415429BOK
CSB-BP415429BOK
CSB-MP415429BOK
CSB-EP415429BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Pantothenate synthetase (panC) CSB-YP415430BOK
CSB-EP415430BOK
CSB-BP415430BOK
CSB-MP415430BOK
CSB-EP415430BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Small, acid-soluble spore protein H 2 (sspH2) CSB-YP415431BOK
CSB-EP415431BOK
CSB-BP415431BOK
CSB-MP415431BOK
CSB-EP415431BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis NH (3)-dependent NAD (+) synthetase CSB-YP415432BOK
CSB-EP415432BOK
CSB-BP415432BOK
CSB-MP415432BOK
CSB-EP415432BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0316 protein Bcer98_2136 (Bcer98_2136), partial CSB-YP415433BOK1
CSB-EP415433BOK1
CSB-BP415433BOK1
CSB-MP415433BOK1
CSB-EP415433BOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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