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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 30S ribosomal protein S20 (rpsT) CSB-YP414614SUK
CSB-EP414614SUK
CSB-BP414614SUK
CSB-MP414614SUK
CSB-EP414614SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uridine kinase (udk) CSB-YP414615SUK
CSB-EP414615SUK
CSB-BP414615SUK
CSB-MP414615SUK
CSB-EP414615SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidine--tRNA ligase (hisS) CSB-YP414616SUK
CSB-EP414616SUK
CSB-BP414616SUK
CSB-MP414616SUK
CSB-EP414616SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP414617SUK
CSB-EP414617SUK
CSB-BP414617SUK
CSB-MP414617SUK
CSB-EP414617SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Valine--tRNA ligase (valS), partial CSB-YP414618SUK
CSB-EP414618SUK
CSB-BP414618SUK
CSB-MP414618SUK
CSB-EP414618SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L35 (rpmI) CSB-YP414619SUK
CSB-EP414619SUK
CSB-BP414619SUK
CSB-MP414619SUK
CSB-EP414619SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Septation ring formation regulator EzrA (ezrA), partial CSB-YP414620SUK
CSB-EP414620SUK
CSB-BP414620SUK
CSB-MP414620SUK
CSB-EP414620SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (guanine-N (7)-)-methyltransferase CSB-YP414621SUK
CSB-EP414621SUK
CSB-BP414621SUK
CSB-MP414621SUK
CSB-EP414621SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine synthase (metK) CSB-YP414622SUK
CSB-EP414622SUK
CSB-BP414622SUK
CSB-MP414622SUK
CSB-EP414622SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splC (splC) CSB-YP414623SUK
CSB-EP414623SUK
CSB-BP414623SUK
CSB-MP414623SUK
CSB-EP414623SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Philodryas olfersii Protease inhibitor kunitoxin-Phi1 CSB-YP414624PYL
CSB-EP414624PYL
CSB-BP414624PYL
CSB-MP414624PYL
CSB-EP414624PYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP414625SUK
CSB-EP414625SUK
CSB-BP414625SUK
CSB-MP414625SUK
CSB-EP414625SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Monofunctional glycosyltransferase (mgt), partial CSB-YP414626SUK1
CSB-EP414626SUK1
CSB-BP414626SUK1
CSB-MP414626SUK1
CSB-EP414626SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Philodryas olfersii Lectoxin-Phi2 CSB-YP414627PYL
CSB-EP414627PYL
CSB-BP414627PYL
CSB-MP414627PYL
CSB-EP414627PYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP414628SUK
CSB-EP414628SUK
CSB-BP414628SUK
CSB-MP414628SUK
CSB-EP414628SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP414629SUK
CSB-EP414629SUK
CSB-BP414629SUK
CSB-MP414629SUK
CSB-EP414629SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Philodryas olfersii Waprin-Phi1 CSB-YP414630PYL
CSB-EP414630PYL
CSB-BP414630PYL
CSB-MP414630PYL
CSB-EP414630PYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Philodryas olfersii Waprin-Phi3 CSB-YP414631PYL
CSB-EP414631PYL
CSB-BP414631PYL
CSB-MP414631PYL
CSB-EP414631PYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP414632SUK
CSB-EP414632SUK
CSB-BP414632SUK
CSB-MP414632SUK
CSB-EP414632SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus microlepidotus Long neurotoxin 3FTx-Oxy1 CSB-YP414633OGE
CSB-EP414633OGE
CSB-BP414633OGE
CSB-MP414633OGE
CSB-EP414633OGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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