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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Anaeromyxobacter sp. LexA repressor (lexA) CSB-YP415534AUT
CSB-EP415534AUT
CSB-BP415534AUT
CSB-MP415534AUT
CSB-EP415534AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Elongation factor 4 (lepA), partial CSB-YP415535AUT
CSB-EP415535AUT
CSB-BP415535AUT
CSB-MP415535AUT
CSB-EP415535AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Phosphoglycerate kinase (pgk) CSB-YP415536AUT
CSB-EP415536AUT
CSB-BP415536AUT
CSB-MP415536AUT
CSB-EP415536AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. DNA repair protein recO (recO) CSB-YP415537AUT
CSB-EP415537AUT
CSB-BP415537AUT
CSB-MP415537AUT
CSB-EP415537AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Adenylosuccinate synthetase (purA) CSB-YP415538AUT
CSB-EP415538AUT
CSB-BP415538AUT
CSB-MP415538AUT
CSB-EP415538AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. V-type ATP synthase beta chain (atpB) CSB-YP415539AUT
CSB-EP415539AUT
CSB-BP415539AUT
CSB-MP415539AUT
CSB-EP415539AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Probable rRNA maturation factor (Anae109_2711) CSB-YP415540AUT
CSB-EP415540AUT
CSB-BP415540AUT
CSB-MP415540AUT
CSB-EP415540AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP415541AUT
CSB-EP415541AUT
CSB-BP415541AUT
CSB-MP415541AUT
CSB-EP415541AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP415542AUT
CSB-EP415542AUT
CSB-BP415542AUT
CSB-MP415542AUT
CSB-EP415542AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. D-alanine--D-alanine ligase (ddl) CSB-YP415543AUT
CSB-EP415543AUT
CSB-BP415543AUT
CSB-MP415543AUT
CSB-EP415543AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP415544AUT
CSB-EP415544AUT
CSB-BP415544AUT
CSB-MP415544AUT
CSB-EP415544AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L27 (rpmA) CSB-YP415545AUT
CSB-EP415545AUT
CSB-BP415545AUT
CSB-MP415545AUT
CSB-EP415545AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Probable RNA methyltransferase Anae109_4379 (Anae109_4379) CSB-YP415546AUT
CSB-EP415546AUT
CSB-BP415546AUT
CSB-MP415546AUT
CSB-EP415546AUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Protein hfq (hfq) CSB-YP415547FDS
CSB-EP415547FDS
CSB-BP415547FDS
CSB-MP415547FDS
CSB-EP415547FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Glycogen synthase (glgA) CSB-YP415548FDS
CSB-EP415548FDS
CSB-BP415548FDS
CSB-MP415548FDS
CSB-EP415548FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum tRNA modification GTPase MnmE (mnmE) CSB-YP415549FDS
CSB-EP415549FDS
CSB-BP415549FDS
CSB-MP415549FDS
CSB-EP415549FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP415550FDS
CSB-EP415550FDS
CSB-BP415550FDS
CSB-MP415550FDS
CSB-EP415550FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Ribosome maturation factor RimM (rimM) CSB-YP415551FDS
CSB-EP415551FDS
CSB-BP415551FDS
CSB-MP415551FDS
CSB-EP415551FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Proline--tRNA ligase (proS), partial CSB-YP415552FDS
CSB-EP415552FDS
CSB-BP415552FDS
CSB-MP415552FDS
CSB-EP415552FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Glutamate--tRNA ligase 2 (gltX2) CSB-YP415553FDS
CSB-EP415553FDS
CSB-BP415553FDS
CSB-MP415553FDS
CSB-EP415553FDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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