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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype O:1b Protein smg (smg) CSB-YP416734YAN
CSB-EP416734YAN
CSB-BP416734YAN
CSB-MP416734YAN
CSB-EP416734YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 30S ribosomal protein S11 (rpsK) CSB-YP416735YAN
CSB-EP416735YAN
CSB-BP416735YAN
CSB-MP416735YAN
CSB-EP416735YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 50S ribosomal protein L6 (rplF) CSB-YP416736YAN
CSB-EP416736YAN
CSB-BP416736YAN
CSB-MP416736YAN
CSB-EP416736YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b 30S ribosomal protein S3 (rpsC) CSB-YP416737YAN
CSB-EP416737YAN
CSB-BP416737YAN
CSB-MP416737YAN
CSB-EP416737YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Protein slyX (slyX) CSB-YP416738YAN
CSB-EP416738YAN
CSB-BP416738YAN
CSB-MP416738YAN
CSB-EP416738YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Rhomboid protease glpG (glpG), partial CSB-YP416739YAN1
CSB-EP416739YAN1
CSB-BP416739YAN1
CSB-MP416739YAN1
CSB-EP416739YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP416740YAN
CSB-EP416740YAN
CSB-BP416740YAN
CSB-MP416740YAN
CSB-EP416740YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Protein FdhD homolog (fdhD) CSB-YP416741YAN
CSB-EP416741YAN
CSB-BP416741YAN
CSB-MP416741YAN
CSB-EP416741YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Small heat shock protein ibpA (ibpA) CSB-YP416742YAN
CSB-EP416742YAN
CSB-BP416742YAN
CSB-MP416742YAN
CSB-EP416742YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP416743YAN
CSB-EP416743YAN
CSB-BP416743YAN
CSB-MP416743YAN
CSB-EP416743YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative septation protein spoVG (spoVG) CSB-YP416744CWU
CSB-EP416744CWU
CSB-BP416744CWU
CSB-MP416744CWU
CSB-EP416744CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP416745CWU
CSB-EP416745CWU
CSB-BP416745CWU
CSB-MP416745CWU
CSB-EP416745CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Leucine--tRNA ligase (leuS), partial CSB-YP416746CWU
CSB-EP416746CWU
CSB-BP416746CWU
CSB-MP416746CWU
CSB-EP416746CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Uracil phosphoribosyltransferase (upp) CSB-YP416747CWU
CSB-EP416747CWU
CSB-BP416747CWU
CSB-MP416747CWU
CSB-EP416747CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein translocase subunit SecA (secA), partial CSB-YP416748CWU
CSB-EP416748CWU
CSB-BP416748CWU
CSB-MP416748CWU
CSB-EP416748CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Adenine deaminase (ade), partial CSB-YP416749CWU
CSB-EP416749CWU
CSB-BP416749CWU
CSB-MP416749CWU
CSB-EP416749CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Asparagine--tRNA ligase (asnS) CSB-YP416750CWU
CSB-EP416750CWU
CSB-BP416750CWU
CSB-MP416750CWU
CSB-EP416750CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional protein FolD (folD) CSB-YP416751CWU
CSB-EP416751CWU
CSB-BP416751CWU
CSB-MP416751CWU
CSB-EP416751CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0251 protein CLB_1397 (CLB_1397) CSB-YP416752CWU
CSB-EP416752CWU
CSB-BP416752CWU
CSB-MP416752CWU
CSB-EP416752CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP phosphoribosyltransferase (hisG) CSB-YP416753CWU
CSB-EP416753CWU
CSB-BP416753CWU
CSB-MP416753CWU
CSB-EP416753CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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