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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Campylobacter jejuni subsp. doylei Adenylosuccinate synthetase (purA) CSB-YP415514CWE
CSB-EP415514CWE
CSB-BP415514CWE
CSB-MP415514CWE
CSB-EP415514CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP415515CWE
CSB-EP415515CWE
CSB-BP415515CWE
CSB-MP415515CWE
CSB-EP415515CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei ATP phosphoribosyltransferase (hisG) CSB-YP415516CWE
CSB-EP415516CWE
CSB-BP415516CWE
CSB-MP415516CWE
CSB-EP415516CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Protein RecA (recA) CSB-YP415517CWE
CSB-EP415517CWE
CSB-BP415517CWE
CSB-MP415517CWE
CSB-EP415517CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 30S ribosomal protein S8 (rpsH) CSB-YP415518CWE
CSB-EP415518CWE
CSB-BP415518CWE
CSB-MP415518CWE
CSB-EP415518CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 50S ribosomal protein L29 (rpmC) CSB-YP415519CWE
CSB-EP415519CWE
CSB-BP415519CWE
CSB-MP415519CWE
CSB-EP415519CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 50S ribosomal protein L23 (rplW) CSB-YP415520CWE
CSB-EP415520CWE
CSB-BP415520CWE
CSB-MP415520CWE
CSB-EP415520CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP415521AUT
CSB-EP415521AUT
CSB-BP415521AUT
CSB-MP415521AUT
CSB-EP415521AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S18 (rpsR) CSB-YP415522AUT
CSB-EP415522AUT
CSB-BP415522AUT
CSB-MP415522AUT
CSB-EP415522AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. UPF0042 nucleotide-binding protein Anae109_0152 (Anae109_0152) CSB-YP415523AUT
CSB-EP415523AUT
CSB-BP415523AUT
CSB-MP415523AUT
CSB-EP415523AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribonuclease HII (rnhB) CSB-YP415524AUT
CSB-EP415524AUT
CSB-BP415524AUT
CSB-MP415524AUT
CSB-EP415524AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Dihydrodipicolinate reductase (dapB) CSB-YP415525AUT
CSB-EP415525AUT
CSB-BP415525AUT
CSB-MP415525AUT
CSB-EP415525AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Proline--tRNA ligase (proS), partial CSB-YP415526AUT
CSB-EP415526AUT
CSB-BP415526AUT
CSB-MP415526AUT
CSB-EP415526AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP415527AUT
CSB-EP415527AUT
CSB-BP415527AUT
CSB-MP415527AUT
CSB-EP415527AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP415528AUT
CSB-EP415528AUT
CSB-BP415528AUT
CSB-MP415528AUT
CSB-EP415528AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP415529AUT
CSB-EP415529AUT
CSB-BP415529AUT
CSB-MP415529AUT
CSB-EP415529AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP415530AUT
CSB-EP415530AUT
CSB-BP415530AUT
CSB-MP415530AUT
CSB-EP415530AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S7 (rpsG) CSB-YP415531AUT
CSB-EP415531AUT
CSB-BP415531AUT
CSB-MP415531AUT
CSB-EP415531AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L15 (rplO) CSB-YP415532AUT
CSB-EP415532AUT
CSB-BP415532AUT
CSB-MP415532AUT
CSB-EP415532AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP415533AUT
CSB-EP415533AUT
CSB-BP415533AUT
CSB-MP415533AUT
CSB-EP415533AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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