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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vanderwaltozyma polyspora Protein YIM1 (YIM1) CSB-YP415874VDW
CSB-EP415874VDW
CSB-BP415874VDW
CSB-MP415874VDW
CSB-EP415874VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Regulator of rDNA transcription 14 (RRT14) CSB-YP415875VDW
CSB-EP415875VDW
CSB-BP415875VDW
CSB-MP415875VDW
CSB-EP415875VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP415876VDW
CSB-EP415876VDW
CSB-BP415876VDW
CSB-MP415876VDW
CSB-EP415876VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Mitochondrial translation factor ATP22 (ATP22), partial CSB-YP415877VDW
CSB-EP415877VDW
CSB-BP415877VDW
CSB-MP415877VDW
CSB-EP415877VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Adenylosuccinate synthetase (Kpol_1054p43) CSB-YP415878VDW
CSB-EP415878VDW
CSB-BP415878VDW
CSB-MP415878VDW
CSB-EP415878VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Probable kynurenine formamidase (BNA7) CSB-YP415879VDW
CSB-EP415879VDW
CSB-BP415879VDW
CSB-MP415879VDW
CSB-EP415879VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (PRP5), partial CSB-YP415880VDW
CSB-EP415880VDW
CSB-BP415880VDW
CSB-MP415880VDW
CSB-EP415880VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Type 1 phosphatases regulator YPI2 (YPI2) CSB-YP415881VDW
CSB-EP415881VDW
CSB-BP415881VDW
CSB-MP415881VDW
CSB-EP415881VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ATP-dependent RNA helicase DBP8 (DBP8) CSB-YP415882VDW
CSB-EP415882VDW
CSB-BP415882VDW
CSB-MP415882VDW
CSB-EP415882VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Defect at low temperature protein 1 (DLT1), partial CSB-YP415883VDW
CSB-EP415883VDW
CSB-BP415883VDW
CSB-MP415883VDW
CSB-EP415883VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Protein SWT21 (SWT21) CSB-YP415884VDW
CSB-EP415884VDW
CSB-BP415884VDW
CSB-MP415884VDW
CSB-EP415884VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora NADH-cytochrome b5 reductase 2-B (MCR1B), partial CSB-YP415885VDW1
CSB-EP415885VDW1
CSB-BP415885VDW1
CSB-MP415885VDW1
CSB-EP415885VDW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora rRNA biogenesis protein RRP36 (RRP36) CSB-YP415886VDW
CSB-EP415886VDW
CSB-BP415886VDW
CSB-MP415886VDW
CSB-EP415886VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Phosphatidylethanolamine N-methyltransferase 1 (CHO2-1), partial CSB-YP415887VDW
CSB-EP415887VDW
CSB-BP415887VDW
CSB-MP415887VDW
CSB-EP415887VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Biogenesis of lysosome-related organelles complex 1 subunit VAB2 (VAB2) CSB-YP415888VDW
CSB-EP415888VDW
CSB-BP415888VDW
CSB-MP415888VDW
CSB-EP415888VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ATPase expression protein 1, mitochondrial (AEP1) CSB-YP415889VDW
CSB-EP415889VDW
CSB-BP415889VDW
CSB-MP415889VDW
CSB-EP415889VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Genetic interactor of prohibitins 3, mitochondrial (GEP3), partial CSB-YP415890VDW
CSB-EP415890VDW
CSB-BP415890VDW
CSB-MP415890VDW
CSB-EP415890VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Amino-acid acetyltransferase, mitochondrial (ARG2), partial CSB-YP415891VDW
CSB-EP415891VDW
CSB-BP415891VDW
CSB-MP415891VDW
CSB-EP415891VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Regulator of rDNA transcription protein 5 (RRT5) CSB-YP415892VDW
CSB-EP415892VDW
CSB-BP415892VDW
CSB-MP415892VDW
CSB-EP415892VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Restriction of telomere capping protein 1 (RTC1), partial CSB-YP415893VDW
CSB-EP415893VDW
CSB-BP415893VDW
CSB-MP415893VDW
CSB-EP415893VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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